摘要
首次在国内以蛋白核小球藻 (Chlorella pyrenoidosa)为模式生物对 2 4种除草剂进行了生物筛选试验 ,结果表明 ,用蛋白核小球藻筛选除草剂活性的方法快速、敏感 ,恰好弥补了培养皿法的不足之处 ,可以构成除草剂活性筛选的第二套筛子。用这种方法对化合物进行常规筛选 ,将减少具有潜在活性化合物的漏筛 ,也可作为初筛以适应微型高通量筛选的要求。
The biological screening for 24 herbicides in 15 different chemical classes with 9 different functions using Chlorella pyrenoidosa as a model biology, shows that this method for herbicide screening is very quick, simple,sensitive and more perfect than the Petri dishes method.Thus, the in vitro system can offer a second screening sieve to ensure that new potential lead structures will not be lost. Moreover, a set of biotests using Chlorella pyrenoidosa cell suspensions is suggested to form a herbicide prescreen in order to eliminate inactive compounds before the more expensive greenhouse screen on higher plants. The method has been shown to be suitable for the requirements for a microscreen and high throughput screening in vitro (HTS in vitro )
出处
《农药学学报》
CAS
CSCD
2000年第2期29-34,共6页
Chinese Journal of Pesticide Science
基金
国家南方农药创制中心!(No.96 - A0 2 - 0 3)
