摘要
目的 研究雌激素受体 (ER)α、ERβ和断裂的雌激素受体产物 1(TERP 1)在乙酚(DES)诱发的大鼠催乳素瘤中的表达。方法 雌性Wistar大鼠摘除卵巢后皮下植入含DES 2 0mg的硅胶管 ,以空白硅胶管作对照。 8周后处死动物 ,用放免法测定大鼠血清催乳素水平 ,观察大鼠垂体组织学改变 ,并用免疫组化方法检测催乳素染色阳性细胞 ,用逆转录 聚合酶链反应分析垂体组织中ERα、ERβ和TERP 1mRNA的转录水平。 结果 实验组大鼠血清催乳素水平、垂体重量和垂体组织催乳素阳性细胞计数均明显高于对照组 (P <0 .0 0 1) ,ERα、ERβ和TERP 1mRNA在两组大鼠垂体组织中均有转录 ,其中ERα和TERP 1mRNA在实验组中的转录水平比在对照组高 (P <0 .0 0 1和P <0 .0 5 )。结论 雌激素可以通过雌激素受体直接影响垂体催乳素细胞。正常的雌激素受体与异常的雌激素受体的共同存在提示雌激素调节作用的异质性 ,它们之间的相互关系及与垂体肿瘤发生的关系 ,有待于进一步阐明。
Objective To examine the expressions of estrogen receptor α (ERα), estrogen receptor β (ERβ) and pituitary specific truncated estrogen receptor product 1 (TERP 1) in diethylstilbestrol (DES) induced prolactinoma of rats. Methods Ovariectomised Wistar rats were subcutaneously implanted with an implant containing 20 mg DES. Rats were implanted with a blank implant as controls. Eight weeks later, serum prolactin level of rats was measured by RIA. Prolactin immunoreactive cells of anterior pituitary were detected by immunohistochemistry assay. ERα, ERβ and TERP 1 mRNAs in pituitaries were examined by RT PCR. Results Serum prolactin levels, pituitary weights and pituitary prolactin immunoreactive cell countings in experimental group rats were all obviously higher than those in control group (P<0.001 respectively). ERα, ERβ and TERP 1 mRNAs were all transcripted in DES induced rat prolactinomas. ERα and TERP 1 mRNA levels were significantly higher in experimental group than those in control group (P<0.001 and P<0.05). Conclusion Estrogen plays a direct role in regulating pituitary prolactin cells through estrogen receptors. Further functional studies will be required to determine whether coexpression of ER variants along with normal ER confers a pathophysiological role in pituitary hormone regulation and (or) tumor cell proliferation.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2001年第1期47-50,共4页
Chinese Journal of Endocrinology and Metabolism
