摘要
目的：探讨用乙菧酚（ＤＥＳ）诱发Ｗｉｓｔａｒ大鼠垂体泌乳素（ＰＲＬ）瘤形成的规律和发病机制，及山莨菪碱和孕酮的预防作用。方法：雌性Ｗｉｓｔａｒ大鼠切除卵巢后随机分为４ 组：①对照组（ｎ＝ １０）：在项背部皮下植入空白植入子；②山莨菪碱组（ｎ＝ １０）：皮下同时植入山莨菪碱１０ ｍ ｇ 和ＤＥＳ２０ ｍ ｇ 两种植入子；③孕酮组（ｎ＝ １０）：皮下植入含１０ ｍ ｇ 孕酮和２０ ｍ ｇ ＤＥＳ两种植入子；④ＤＥＳ组（ｎ＝ ２５）：皮下植入含２０ ｍ ｇ ＤＥＳ植入子。分别于第２ 至第１０ 周处死收集躯干血标本，分离血清测定ＰＲＬ，同时摘除垂体前叶，分别做匀浆测定ＰＲＬ及ＰＲＬ免疫组化检查。切除下丘脑，匀浆测定多巴胺（ＤＡ）和去甲肾上腺素（ＮＥ）水平。结果：ＤＥＳ组大鼠的垂体重量以及垂体和血清ＰＲＬ水平从第２ 周起显著升高（Ｐ＜ ０．０１ 或＜ ０．０５）。ＤＥＳ组大鼠垂体ＰＲＬ细胞在第２ 周和第４ 周呈现增生性改变，第６ 周部分呈腺瘤性改变，第８ 周和１０ 周全部为腺瘤样改变。下丘脑的ＤＡ和ＮＥ浓度于第４ 周下降到最低点，和对照组大鼠比较差异非常显著（Ｐ均＜ ０．０１）。山莨菪碱组和孕酮组的各项指标和ＤＥＳ组比较差异无显著性意义（Ｐ均＞ ０．?
Objective:To investigate the forming regularity and pathogenesis of DES induced pituitary prolactinoma and the preventing effects of anisodamine and progesterone. Methods:Ovariectomized female Wistar rats were randomly divided into 4 groups: (1) Control group ( n =10): A blank implant was subcutaneously implanted over the neck; (2) Anisodamine group ( n =10): An implant contained 10 mg anisodamine and an implant with 20 mg DES were subcutaneously coimplanted; (3) Progesterone group ( n =10); 10 mg progesterone implant and 20 mg DES implant were co implanted; (4) DES group ( n =25): 20 mg DES implant only. Five rats in DES group were executed in Week 2、 4、 6、 8 and 10 respectively, and 5 rats in the remaining 3 groups were executed in week 8 and 10 respectively. Trunk blood sample were collected for serum PRL measurement. The anterior pituitary gland and hypothalamus were extirpated for PRL immunocytochemical examination and dopamine (DA) and norepinephrine (NE) measurement. Results:The weight of pituitary gland and serum and pituitary PRL levels in rats of the DES group were significantly greater than those of control group (all P <0 01 or <0 05). Pituitary gland during week 2 and 4 presented lactotropic hyperplasia, and partial pituitary gland became prolactinoma in week 6, and all of them developed prolactinoma in week 8 and 10. The DA and NE concentrations in hypothalamus decreased to the nadir in week 4 and were significantly lower than those of control group (all P <0 01). All the parameters mentioned above in anisodamine group and progesterone group were not signifiantly different to DES group (all P >0 05). Conclusion:(1) Subcutaneous DES implantation is capable of stimulating the lactotrope hyperplasia and finally forming prolactinoma in Wistar rat; (2) The decrease of hypothalamic DAand NE concentrations in DES group suggestes that hypothalamic dopaminergic and noradrenergic neurons take part in the regulation of PRL secretion; (3) The effect of progesterone protecting lactotropes against DES induced prolactinoma was not found; and (4) For no effect of amisodamine on preventing DES induced pituitary prolactinoma, the hypothalamic cholinergic neurons seem not to joint the regulation of pituitary PRL secretion.
出处
《军医进修学院学报》
1999年第4期263-266,共4页
Academic Journal of Pla Postgraduate Medical School
