摘要
背景与目的:探讨雌激素诱发大鼠垂体催乳素(PRL)瘤的发病机制。材料与方法:制备雌激素诱发大鼠PRL瘤模型:成年雄性Wistar大鼠16只,随机分为对照组(n=8)和PRL瘤组(n=8)。对照组皮下植入空白硅胶管,PRL瘤组皮下植入含有乙烯雌酚的硅胶管,用药8周后乙醚麻醉大鼠,心脏穿刺取血,分离血清,并开颅摘取垂体。采用放免法测定大鼠血清PRL水平;垂体称重并做组织病理学观察;用免疫组织化学方法检测垂体组织PRL蛋白的表达和分布;用RT_PCR方法检测c_fos和垂体肿瘤转化基因(PTTG)在PRL瘤组织中的表达,计算机凝胶成像系统分析其表达量。结果:雌激素作用8周后,根据大鼠垂体重量以及组织学和免疫组化的改变,证实已诱发出PRL瘤模型。在PRL瘤组中,c_fos和PTTG的表达量均明显高于对照组,二者差异具有统计学意义彩(P<0.001)。结论:雌激素刺激垂体PRL细胞表达癌基因c_fos以及PTTG,在雌激素诱发大鼠PRL细胞增生以至最终形成PRL瘤的过程中起一定的作用。
BACKGROUND ＆ AIM： To study the mechanism of estrogen in an estrogen-induced prolactinoma model. MATERIALS AND MEthODS： ① Preparation of rat prolactinoma model： adult male Wistar rats were divided into 2 groups at random. Each rat in control group（n = 8）received a subcutaneous blank implant. Rats in prolactinoma group（n =8） received estrogen-containing implants. After 8 weeks, all the animals were sacrificed. ② Each pituitary gland was weighed and collected for histopathological and immunocytochemical examination. ③ Serum prolactin（PRL） levels were measured by RIA method. ④ Both c-fos mRNA level and pituitary tumor transforming gene （PTTG） mRNA level in pituitary tissue were measured by RT-PCR method. RESULTS： Prolactinoma model were prepared successfully as reflected by serum PRL level, pituitary weight and immunocytochemical examination. The expression levels of both c-fos mRNA and PTTG mRNA in prolactinoma group were obviously higher than those in control group（P 〈 0.001）. CONCLUSION： Estrogen stimulated the expression of c-fos and PTTG oncogenes, which played important roles in estrogen-induced prolactinoma model.
出处
《癌变·畸变·突变》
CAS
CSCD
2006年第6期435-438,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助项目(No.20137010)
首都医学发展科研基金(中医药类)资助项目(编号2001-Ⅲ-21)
