摘要
目的 探讨Ki 6 7抗原表达与原发性胆囊癌病理生物学行为的关系。方法 采用免疫组化S P法 ,检测胆囊良、恶性病变中Ki 6 7抗原的表达情况 ,以其表达阳性率评估胆囊癌组织的增生活性。结果 Ki 6 7抗原在胆囊癌组织中的表达量显著高于胆囊良性病变 (P <0 .0 1)。但Ki 6 7抗原的表达强度与胆囊癌组织学类型、Nevin分期及病理分级无明显相关性 (P >0 .0 5 ) ,与胆囊癌的突变型p5 3基因产物表达量呈明显正相关 (P <0 .0 5 )。 结论 Ki 6 7抗原作为 1个代表增殖活性的肿瘤标志 ,其过量表达是胆囊癌高度恶性的生物学行为的客观指标之一 ,对胆囊恶性肿瘤的病理诊断有较高的实用价值。p5 3基因可能通过基因突变增强胆囊癌细胞的增殖活性 ,从而使Ki 6 7抗原表达量明显增加。
Objective To evaluate the relationship between expression of Ki 67 antigen and pathobiological behaviours of primary gallbladder cancers.Methods The expression of Ki 67 antigen in benign and malignant lesion were studied by immunohistochemical method.Results In gallbladder carcinoma,the expression rate of Ki 67 antigen was significantly higher than in benign lesion( P <0.05).No correlation was found between the expression of Ki 67 and the Nevin stages,histological type and pathologicgrades of gallbladder carcinoma.Furthermore,the Ki 67 expression was positively related to the expression level of mutation p53 gene( P <0.05).Conclusion Overexpression of Ki 67 may be an objective marker of the high malignant biological behavior of gallbladder carcinoma.It is valuable for pathological diagnosis of gallbladder carcinoma.The overexpression of Ki 67 in gallbladder carcinoma cells may be caused by the mutation of p53 gene.
出处
《实用癌症杂志》
2001年第1期44-45,69,共3页
The Practical Journal of Cancer
