摘要
目的探讨TLR4/NF-κB信号通路调控铁调素在非酒精性脂肪肝发病中的作用机制。方法 60只SD大鼠,随机分为正常组、模型组和干预组。模型组予高脂饮食制备NAFLD大鼠模型,以TLR4/NF-κB信号通路抑制剂腹腔注射干预组大鼠。处死各组大鼠,观察各组肝组织病理变化,检测TLR4、NF-κB蛋白的表达及铁调素mRNA水平的表达。结果模型组大鼠肝脏呈现典型脂肪性变,其TLR4、NF-κB蛋白表达及铁调素mRNA水平显著高于正常组(P<0.05)。干预组大鼠肝组织病理改变显著改善,TLR4、NF-κB蛋白表达及铁调素mRNA水平显著下降(P<0.05)。结论 TLR4/NF-κB信号通路的异常激活可能上调铁调素表达,参与了非酒精性脂肪肝的发生。
Objective To investigate the mechanism of Hepcidin in non-alcoholic fatty liver disease （NAFLD） adjusted by TLR4/NF-kB signal pathway. Methods Sixty SD rats were divided into normal, model and intervention group. A NAFLD rat model was created using high-fat diet fed. Pathenolide was injected intraperitoneally in the intervention group. Pathological changes of hepatic tissues were observed under microscope. The expression of TLR4 and NF-KB protein in hepatic tissues and the expression of hepcidin mRNA was determined by RT-PCR. Results The typical fatty degeneration occurred in the livers of rats in the model group. The levels of TLR4 and NF-KB protein and hepcidin mRNA were significantly higher than those in the normal group（P 〈 0.05）. The pathologic change of liver tissues of rats in the intervention group was improved obviously,the levels of TLR4 and NF-KB protein and hepcidin mRNA decreased significantly（ P 〈 0. 05 ）. Conclusion The abnormal activation of TLR4/NF-KB signal pathway may be responsible for the up regulation of Hepcidin expression and the occurrence of NAFLD.
出处
《中华全科医学》
2014年第3期384-385,F0003,共3页
Chinese Journal of General Practice
