摘要
目的:建立药对甘草附子总皂苷的含量测定方法。方法:用香草醛-高氟酸试剂,在560 nm处有最大吸收,并时比色条件进行了优化。结果:该方法能较为准确的测定出药对中总皂苷的含量;总皂苷的浓度在0.034~0.102 mg·ml^(-1)范围内呈良好的线性关系(r=0.999 2),平均回收率100.2%,RSD=2.7%(n=6)。结论:该方法灵敏,准确可靠,重复性好,可作为药对甘草附子中总皂苷控制的方法。
Objective： To establish a determination method for total saponins in the drug pair of licolice and aconite. Methods： A colorimetry method was used to determine the total saponins at 560 nm using vanilin-perchloric acid as the reagent. Results： The cali- bration range of glycyrrhizin acid was 0. 034-0. 102 mg·ml^-1 （r =0. 999 2）. The mean recovery was 100.2% and RSD was 2.7% （n = 6）. Conclusion： The method is simple, rapid, accurate and reproducible.
出处
《中国药师》
CAS
2014年第1期93-95,共3页
China Pharmacist
关键词
甘草
附子
总皂苷
药对
分光光度法
Radix glycyrrhizae
Radix aconiti lateralis preparata
Total sapoins
Drug pair
Spectrophotometry
