摘要
血管内皮细胞具有一系列重要生理功能 ,内皮细胞受外界刺激时可转录众多的mRNA ,体现了它们的生物学重要性 .内皮细胞的极早期应答基因 (Immediateearlyresponsegene ,ERG)是一类重要的介导基因 ,决定了外界刺激条件下细胞的最后反应模式 .以包含 40 96条各种人体基因的DNA芯片观察了血管内皮细胞受分化诱导剂PMA(phorbolmyristateacetate)激活后早期应答基因的表达谱 ,证实 17条ERG属已知基因 .数据处理聚类结果表明 ,多数ERG属蛋白质激酶及转录调控因子 ,2 3条上调新基因从功能上归类为ERG ;并且报道了其中的一条新基因的序列特征及其染色体位置和基因结构 ,为研究这个基因的功能提供了有力的实验依据 .
Endothelial cells play important physiological roles, the identity and abundance of mRNA species within endothelial cells indicate their biological potential. The induction of immediate early responses genes (ERG) in endothelials is critical to determining the ultimate outcome of exposure to mitogen. The response of endothelial cells to PMA, was explored with a complementary DNA microarray representing about 4 096 different human genes. Interestingly, most of the known genes (13/17) upregulated by PMA are involved in signal transduction or kinases. 23 new genes are also upregulated and belong to ERG functionally. The sequence character, chromosome location and structure of one of the upregulated new genes are rsported, providing compelling experimnetal evidence to further research of this gene's function.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2000年第6期651-655,共5页
Journal of Fudan University：Natural Science
基金
上海现代生物与新药产业发展基金资助项目!(98431912 1)
国家科技部科技型中小企业创新基金资助项目!(0 0C2 6 2 1310 0 16 5 )
