摘要
用基因芯片分析肥厚性疤痕与正常皮肤成纤维细胞在静息及TGF beta3刺激下的表达谱的改变 .正常成纤维细胞在TGF beta3刺激前后的表达谱与肥厚性疤痕成纤维细胞在TGF beta3刺激前的表达谱正相关 (r=0 .76 0 37) ,刺激后 2种成纤维细胞的表达差异与肥厚性疤痕成纤维细胞TGF beta3刺激前后的表达谱差异正相关 (r =0 .82 6 76 2 ) .构成以上差异的基因有 6 2 0条 ,涉及细胞骨架蛋白、细胞周期蛋白、细胞因子、受体、转录因子及糖、蛋白质和核酸代谢的酶类 ;未发现胶原蛋白Ⅰ ,Ⅲ的表达水平的改变 .结果提示 :1.肥厚性疤痕成纤维细胞在静息状态下表现出与正常细胞受TGF beta3刺激后相似的反应 ,其可能处于一种应激状态 ;2 .TGF beta3刺激后 2种细胞的反应差异与肥厚性疤痕细胞的异常反应相关 ,TGF beta3的作用背景可能是肥厚性疤痕的分子病理学基础之一 ;3 .基因表达变化涉及细胞因子、细胞周期调控、凋亡等 ,其具备疾病遗传异质性的分子基础 ;4.胶原表达无明显上升 ,提示在肥厚性疤痕发病中 ,胶原纤维及细胞外基质堆积有复杂的分子生物学基础 .
The expression fingerprint of HS fibroblasts was analyzed by cDNA microarray, using mRNA derived from multiple tissues as a contrast. The expression fingerprint of HS fibroblasts was similar to that of normal fibroblast post stimulated by TGF beta3. The expression pattern between the HS and the normal fibroblasts post stimulated was correlated to expression pattern between HS fibroblasts prior and post stimulated by TGF beta3. The expression of 620 genes related to cell cycle, cytokine, oncoprotein and apoptosis was up regulated or down regulated at least 3 times. However, the expression of type Ⅰ and Ⅲ collagen was not changed. The results suggest: (1)The HS fibroblast was in a stress status; (2) Over expression of collagen type Ⅰ and Ⅲ was not necessary for HS formation.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2000年第6期634-638,644,共6页
Journal of Fudan University：Natural Science
基金
上海现代生物与新药产业发展基金资助项目!(98431912 1)
