摘要
通过对构建的人胎脑cDNA文库进行大规模测序 ,发现一条人类新基因 ,序列与仓鼠asr2序列高度同源 ,达 88% ,命名为人类抗砷相关基因 (humanarsenicresistancerelatedgene ,hARRG) .hARRG与抗砷基因相关序列ARS2 (AF0 82 871)几乎完全同源 ,仅比它短 2 5 3bp .hARRG并不是全长基因而仅是一个cDNA片段 .为了获得hARRG全长cDNA ,运用Northernblot ,电子RACE和 5′ SMART RACE的方法克隆了一条 2 999bp的hARRG全长cDNA ,通过Unigene染色体定位于 7q2 1.3,经过生物信息学分析发现 ,该cDNA有完整的读码框 ,编码一个 788个氨基酸的蛋白质 .预计分子质量为 89 2ku ,并在大肠杆菌中获得了成功表达 .
A novel human full length cDNA was cloned from a fetal brain cDNA library through a high throughput cDNA sequencing project. It showed 88 % identity in nucleic acid sequence to asr 2 of hamster. It was named human arsenite resistance related gene ( hARRG ). Moverover hARRG was almost completely identity to ARS 2 (GenBank No. AF082871) except that hARRG was 253 bp shorter than ARS 2. It seems not a full length gene. A full length hARRG cDNA of 2 999 bp was cloned with Northern blot, electron RACE and 5′ SMART RACE. This cDNA is located in chromosome 7q21.3 by Unigene. It has a integrated ORF, encoding a protein of 788 amino acids, with putative molecular weight of 89.2 ku. The ORF of hARRG was subcloned into reconstructed PET11c plasmid and found expressed in E. coli . with IPTG inducing.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2000年第6期623-627,共5页
Journal of Fudan University：Natural Science
