摘要
应用高效液相色谱(HPLC)法建立桂枝茯苓丸的指纹图谱。采用ANGLA Promosil C18色谱柱(250×4.6mm,5μm),以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,230nm检测,优化条件下,桂枝茯苓丸各成分得到较好的分离。经方法学验证,方法具良好的精密度、重复性和稳定性。采用中药色谱指纹图谱相似度评价系统软件(2004A版)进行指纹图谱分析,11批桂枝茯苓丸样品相似度均在0.90以上。实验表明:桂枝茯苓丸的HPLC指纹图谱特征性和专属性强,可较系统地用于桂枝茯苓丸的质量控制。
The fingerprint of Guizhi Fuling pill was est ts of Guizhi Fuling pill were separated e wavelength of 230 nm and a gradient ablished by high ffectively on a program with to the similarity evaluation system for chromatog （Version 2004 A） the similarity of f higher than 0.90. The fingerprint co the research of effective components ingerpint of uld be used 11 batches as a metho of Guizhi Fuling Pill. performance liquid ANGI.A acetomt chromatography. The promosid C18 column with a rile-0. 1% phosphoric acid. raphic fingerprint of chinese of Guizhi Fuling pill decocti materia medica on d of quality control and provide samples was evidence for the research of effective components of Guizhi Fuling Pill.
出处
《分析科学学报》
CAS
CSCD
北大核心
2014年第1期100-102,共3页
Journal of Analytical Science
基金
黑龙江教育厅骨干教师项目(1251G057)
关键词
桂枝茯苓丸
高效液相色谱
指纹图谱
Guizhi Fuling pill
High performance liquid chromatography
Fingerprint chromatography
