摘要
采用RT-PCR技术探究盐生植物费尔干猪毛菜病程相关蛋白基因SfPR-1(GenBank登录号:JQ670917)在不同发育时期、组织部位、植物激素、非生物胁迫及生物胁迫处理下的表达规律,以揭示该基因在费尔干猪毛菜生长发育和逆境胁迫下的作用。结果表明,不同组织(根、茎、叶)中,SfPR-1在根中表达量最高,预示该基因可能在根防御中发挥主要作用;SfPR-1在不同发育阶段(种子、种子萌发20 d幼苗、种子萌发30 d幼苗、种子萌发40 d幼苗)的表达特性显示,种子萌发30 d幼苗时,其表达差异最显著,表明其可能在植株后期生长发育中具有重要作用;SfPR-1对不同植物激素(水杨酸SA、茉莉酸JA、脱落酸ABA、乙烯合成前体ACC)均产生了响应,表明该基因在几条主要的抗病防御通路中均发挥作用。非生物胁迫(H2O2、盐、旱、冷)都能够诱导SfPR-1基因的表达,其中对盐响应程度最高。以植源性意大利青霉(Penicillium italicum)进行生物胁迫时,SfPR-1表达呈持续上升趋势。以上结果表明费尔干猪毛菜病程相关蛋白基因SfPR-1是生物与非生物逆境胁迫下均响应的基因,推测其在植物抵御逆境胁迫中发挥重要作用。
To investigate the potential roles of SfPR-1 at different developmental stages, tissues, phytohormones treatment, different abiotic stresses and biotic stress treatment, semi-quantitative RT-PCR method was employed to explore the expression profiles of pathogenesis- related protein gene SfPR-1 （ GenBank accession number ： JQ670917 ） from halophytes Salsolaferganica. The results displayed that SfPR-I gene was mainly expressed in root tissue with low expression in the stem and leaves, suggesting that the gene may play a major role in the root defense. The expression characteristic of SfPR-1 at different developmental stages （ seed, 20 d shoots after seed germination, 30 d shoots after seed germination, 40 d shoots after seed germination ） showed the highest level was in 30 d shoots after seed germination, indicating that may play an important role in the plant late growth. The expression of SfPR-] generated with the application of phytohormones including salicylic acid （ SA ）, jasmonate （ JA ）, 1-aminocyelopropane-l-carboxylate （ ACC ）, and abscisic acid （ ABA ） . This response can also be induced by abiotic stresses including H202, salt, dronght, cold, and biotic stresses such as Penicillium italicum infection. The transcript levels of SfPR-1 substantially increased during biotic stress during 48 h treatment. These results suggested that SfPR-lwas a key gene to the biotie and abiotic response and may play an important role in host plant defense and environmental stress.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第1期116-124,共9页
Biotechnology Bulletin
基金
"973"计划前期研究专项(2012CB722204)
新疆自治区青年自然科学基金项目(2012211B06)
