摘要
目的:研究姜黄醇提物对血管性痴呆模型大鼠的保护作用。方法:线拴法复制大鼠血管性痴呆模型,进行神经功能缺失评分。60只SD大鼠均分为假手术(等容生理盐水)组、模型(等容生理盐水)组、尼莫地平(80 mg/kg)组与姜黄醇提物高、中、低剂量(200、100、50 mg/kg)组。灌胃给药,于术前48 h开始给药,每8 h 1次,连续7次。穿梭箱实验测定大鼠主动逃避(AAR)次数、被动逃避(PAR)电击时间;水迷宫实验测定大鼠寻台潜伏期;酶联免疫吸附法测定一氧化氮(NO)、过氧化脂酶(LPO)的含量。结果:与假手术组比较,模型组大鼠神经功能缺失评分升高、AAR次数减少、PAR电击时间延长、寻台潜伏期延长,脑皮质NO、LPO含量增加,差异有统计学意义(P<0.01);与模型组比较,姜黄醇提物高、中剂量组大鼠神经功能缺失评分降低、AAR次数增加、PAR电击时间缩短,寻台潜伏期缩短,脑皮质NO、LPO含量减少,差异有统计学意义(P<0.01或P<0.05)。结论:姜黄醇提物对血管性痴呆模型大鼠有一定保护作用。
OBJECTIVE： To study the effects of Curcuma longa extract on vascular dementia rats. METHODS： The vascular dementia model was induced by suture method, and neurological deficits score was conducted. 60 SD rats were divided into sham operation group（constant volume of normal saline）, model group （constant volume of normal saline）, nimodipine group （80 mg/ kg） and C. longa alcohol extract high-dose, middle-dose and low-dose groups （200,100,50 mg/kg）. They were given relevant medicines intragastrically 48 h before operation every 8 h for consecutive 7 times. The number of active avoidance （AAR） and the time of passive avoidance （PAR） were determined by shuttle box test; the latency of seeking platform （SPL） in rats was determined by water maze test. The contents of NO and LPO were detected by ELISA. RESULTS： Compared with sham operation group, neurological deficits score of model group was increased; the number of AAR was decreased while the time of PAR and SPL were pro- longed; the contents of NO and LPO in pallium of rats were increased; there was statistical significance（P〈0.01）. Compared with model group, neurological deficits scores of C. longa alcohol extract high-dose and middle-dose groups were decreased; the number of AAR was increased while the time of PAR and SPL were shortened; the contents NO and LPO in pallium of rats were decreased significantly（P〈0.01 or P〈0.05）. CONCLUSIONS： C. longa alcohol extract can protect against vascular dementia model rat to some extent.
出处
《中国药房》
CAS
CSCD
2014年第7期596-598,共3页
China Pharmacy
