摘要
目的探讨不同拓扑结构的聚甲基丙烯酸甲酯(polymethylmethacrylate,PMMA)电纺纳米纤维对于PC12细胞神经突生长能力及生长方向的影响。方法构建具有随机或有序拓扑结构的PMMA电纺纳米纤维并评估其生物相容性;利用PMMA薄膜作为对照,利用慢病毒技术转染绿色荧光蛋白基因作为显色手段,分析PC12细胞在纤维支架上的神经突生长能力和生长方向,以及神经突与纤维结构的依存关系。结果 PMMA电纺纤维具有良好的生物相容性,PC12细胞能够在电纺纤维上顺利贴壁并分化;在PMMA薄膜、随机或有序纤维系统上,PC12分化生成的神经突的长度分别为(60.2±29.9)、(64.1±32.5)、(85.7±39.8)μm,和培养在薄膜及随机纤维上的细胞相比,培养在有序纤维上的PC12细胞能够分化出长度更长、且与纤维延伸方向一致的神经突(P<0.01);相比随机电纺纤维,有序纳米纤维对PC12神经突更具引导作用。结论有序电纺纳米纤维具有作为神经损伤后植入性细胞支架的潜力。
Objective To evaluate the influence of electrospun poly（methyl methacrylate） （PMMA） nanofibers with different topographic features on neurite growth and orientation of PC12 cells. Methods Electrospun PMMA nanofibers with random or aligned topographic features were produced and PC12 cells were cultured on them. The biocompatibility of the fibers was evaluated. With PMMA film as control, the neurite growth ability and orientation of PC12 cells were assayed using lentivirus-mediated green fluorescent protein transfection.Results The electrospun PMMA nanofibers had good biocompatibility, and could exert contact guidance to the neurite growth of PC12 cells. On aligned nanofibers, PC12 cells could form neuritis with greater length and better alignment, as compared to random nanofibers and films （length： 85.7±39.8 vs 64.1±32.5 vs 60.2±29.9 μm）.Conclusion Aligned electrospun PMMA nanofibers can be used as a potential cell scaffolding after neural injury.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2014年第4期351-354,共4页
Journal of Third Military Medical University
基金
国家临床重点专科建设项目([2011]170)~~
