摘要
目的：建立同时测定不同产地手掌参药材中多指标成分的质量控制方法，分析气候因子对手掌参分布活性成分含量的影响。方法：采用HPLC，KromasilC18色谱柱（4．6mm×250mm，5μm），以0．1％醋酸水溶液和甲醇为流动相梯度洗脱，流速1．0mL·min-1，检测波长270nm，柱温30℃。结果：4种有效成分的线性关系均良好，精密度、稳定性和重复性的RSD均〈2．50％，平均加样回收率为96．97％-101．55％，RSD均〈1．46％；同时使用Diva—Gis提取不同产地手掌参药材的19个气候数据和3个地理数据进行聚类分析，经与样品有效成分含量测定的计量化学结果比较，表明不同产地手掌参药材各指标成分含量差异较大，通过气候因子的主成分分析和聚类分析，发现其与手掌参的活性成分的含量具有一定的相关性。结论：该方法准确，重复性好，可为科学、全面评价手掌参药材的品质提供参考，对进一步研究手掌参资源的可持续利用具有指导意义。
Objective： To establish a quality control method for simultaneous determination of multi-index component in Gymnadenia conpsea from different regions, and analyze the climatic factors on the impact of G. conpsea＇s active ingredient content. Method： The separation was performed on a Kromasil C18 column （4. 6 mm× 250 mm, 5.0 μm）. Methanol-0. 1% H3PO4 was used as the mobile phase with gradient elution. The flow rate was 1.0 mL .min-l. The detection wavelength was set at 270 nm and the column temperature was maintained at 30 ℃. Result： The linear range was precise. The stability and repeatability were less than 2.50%. The average recoveries were ranged from 96.97% to 101.55% , and the RSD was less than 1.46%. The 19 kinds of climatic factors and 3 kinds of geographic factors were used cluster analysis, Gymnadenia conpsea samples were analyzed by Diva-Gis. Experimental results showed that the index component content from different places of origin had remarkable differences. Meanwhile climatic factors also had relevance participate in the active ingredient content of G. conpsea. Conclusion： This method is accurate and reproducible, which presented a scientific and comprehensive evidence for controlling the quality and using the sustainable of G. conpsea.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第4期65-69,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家大学生创新项目(201210656022)
西南民族大学中央高校基本科研业务费优秀学生培养项目(12ZYXS76)
关键词
手掌参
天麻素
腺苷
对羟基苯甲醇
对羟基苯甲醛
高效液相色谱
气候因子
Gymnadenia conpsea
gastrodin
adenosine
4-hydroxybenzyl alcohol
4-hydroxybenzaldehyde
RP-HPLC
climatic factors
