摘要
目的探讨对羟基苯甲醇抗血小板聚集作用及机制。方法以二磷酸腺苷(ADP)、花生四烯酸(AA)为诱导剂,探讨对羟基苯甲醇的体外抗血小板聚集活性;采用下腔静脉结扎致大鼠静脉血栓方法,考察对羟基苯甲醇的体内抗血小板聚集活性;并利用双波长荧光分光光度法测定对羟基苯甲醇对AA诱导的家兔血小板聚集胞浆钙离子浓度的影响。结果对羟基苯甲醇对AA诱导的家兔体外血小板聚集有明显的对抗作用,其半数抑制率(50% inhibitory concentration,IC50)为0.107 g·L-1,对ADP诱导的家兔体外血小板聚集有抑制作用,IC50为0.3 g·L-1;天麻成分对羟基苯甲醇能显著对抗大鼠下腔静脉血栓的形成;对羟基苯甲醇对AA诱导的家兔血小板细胞外钙内流与内钙释放和对照组相比差异均有非常显著性意义。结论天麻成分对羟基苯甲醇在体外、体内均具有显著的抗血小板聚集活性;其作用机制可能是通过抑制外钙内流和内钙释放达到抑制血小板聚集作用。
Objective To investigate the anti - platelet aggregation funetion of Dihydroxybenzyl alcohol extracted from Gastrodia elata Blume. Methods ADP, AA were used as inducers to study the anti - plateiet aggregation function of Dihydroxybenzyl alco- hol in vitro. Venous thrombosis caused by inferior vena eava ligation method was used to evaluate the anti - platelet aggregation function in vivo. At the same time, the method of Dual - wavelength fluorescence spectrophotometry was used to test the influenee of cytosolic calcium eoncentration. Results Dihydroxybenzyl Aleohol had a significant anti - platelet aggregation function in vitro and the ICso was 0. 107mg ~ ml-l. It also eould significantly inhibit the platelet aggregation induced by ADP in vitro, and the ICso was 0.3rag ~ ml-l ; Dihydroxybenzyl alcohol could significantly inhibit the thrombosis of rat inferior vena cava. Dihydroxybenzyl Alcohol markedly prohibited calcium flowing into and intracellular ealcium releasing compared with control group. Conclusion - Dihydroxybenzyl Aleohol had a significant anti - platelet aggregation function in vitro and in vivo. The mechanism of inhibiting platelet aggregation may through preventing calcium flowing into and intraeellular calcium releasing.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2014年第1期4-6,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.81160514)
教育部科学技术研究重点项目(No.211174)
关键词
天麻
对羟基苯甲醇
抗血小板聚集
血小板胞浆钙离子浓度
Gastrodia elata Blume.
Dihydroxybenzyl Alcohol
Anti -platelet aggregation
Platelet cytosolic caleium concentration
