摘要
采用机械分离及胰蛋白酶和巨型胶原酶联合消化的方法，获得兔髁突软骨细胞。应用ＭＴＴ四唑盐比色法，同位素掺入法检测不同剂量１７－β雌二醇（Ｅ２）（１０－１２～１０－６ｍｏｌ／Ｌ）对兔髁突软骨细胞生长、ＤＮＡ、胶原及蛋白多糖合成的影响，旨在观察雌二醇对体外培养的兔髁突软骨细胞增生与分化的影响。结果表明：１０－１２～１０－８ｍｏｌ／Ｌ及 １０－１１～１０－８ｍｏｌ／Ｌ １７β－Ｅ２对体外培养的兔髁突软骨细胞生长及ＤＮＡ合成具有刺激作用，且呈剂量依赖性，最大效应浓度分别为１０－８ｍｏｌ／Ｌ及 １０－９ｍｏｌ／Ｌ。但进一步增加Ｅ２浓度则刺激作用减弱。１０－６ｍｏｌ／Ｌ具有明显抑制性。Ｅ２对蛋白多糖合成具有相似的作用，最大刺激效应浓度为１０－８ｍｏｌ／Ｌ。而 １０－６ｍｏｌ／Ｌ Ｅ２具有显著抑制作用。Ｅ２对胶原蛋白的合成无明显作用。结果提示：１７β－Ｅ２对体外培养的兔髁突软骨细胞增殖及分化具有双向性作用。随其剂量不同，既具有促进效应又具有抑制效应。表明雌激素对维持关节的正常功能具有重要意义，可能在颞下颌关节某些疾患的发生及进展中起一定作用。
The mandibular condylar cartilage cells were harvested from a neonatal rabbits by dissection and sequential digestion with 0. 25 % trypsin and 0.2 % colloagenase (type Ⅱ). Estradiol (E2) was added into the culture medium at a variety of concentrations. Cell growth, DNA, collagen and proteoglycan synthesis were measured by using MTT method, 3H-thymidine, 3H-proline and 35S-sulfate incorporation in order to inves- tigate the effects of E2 on proliferation and differentiation of mandibular condylar cartilage cells in rabbits in.vit- ro. The results showed that E2 increased cell number and 3H-thymidine incorporation at 10-10 to 10-8 mol/L and 10-11 to 10-8 mol/L in a dose-dependent manner, peaking at 10-8 mol/L and 10-9 mol/L respectively. However, further increase in the concentration of E2 both caused inhibition and the significance was at 10-6 mol/L. The effect of E2 on proteoglycan synthesis was in the similar manner with the maximum stimulating ef- fect being at 10-8 mol/L and inhibition was significant at 10-6 mol/L. E 2 had no effect on 3H-proline incorpo- ration. It was concluded that E2 affected condylar chondrocytes with both stimulating and inhibiting in cell pro- liferation and differentiation in biphasic manner depending upon its concentration. indicating that estrogen might play an important role in the maintenance of normal functions of temporomandibular joint (TMJ) and relevant to some aspects in certain TMJ diseases.
出处
《同济医科大学学报》
CSCD
2000年第6期588-591,共4页
Acta Universitatis Medicinae Tongji
关键词
雌二醇
下颌髁突
软骨细胞
细胞增殖
细胞分化
estradiol
proliferation and differentiation
mandibular condylar cartilage cells
rabbit
