摘要
昆明种雄性小鼠32只,随机分为4组。脱氢表雄酮(DHEA)日摄取量分别为0(对照组)、20、40和60 mg/kg,试验期52 d。各组分别在42日龄、72日龄处死小鼠,分离睾丸,制作石蜡切片,HE染色,显微观察、摄影。结果显示,与对照组比较,42日龄时中、高剂量组的曲精小管断面变形,各时期的生精细胞和成熟精子数量均减少;高剂量组曲精小管内生精细胞层次基本消失,出现较大腔隙,间质发生不同程度的变化。72日龄时中、高剂量组的曲精小管断面变形,基膜变薄伴有断裂和间质受损增加。中剂量组出现畸形精子,高剂量组曲精小管中央空白区域增大,缺乏成熟精子。以上结果表明,中、高剂量的DHEA对生长期小鼠的睾丸组织结构发育有明显的毒性作用。
32 Kunming male mice were randomly divided into 4 groups,which were fed with DHEA of 0 mg/kg （control group）, 20 mg/kg（low-dose group）, 40 mg/kg （medium-dose group） and 60 mg/kg （high-dose group） every day. The experiment lasted for 52 days. The mice in every group were killed on the 42 day-old and 72 day-old respectively. The testis was picked to make into paraffin sections and observe in microscope after HE staining. The results showed that the cross-section of seminiferous tubules deformed on 42 day-old and the number of spermatogenic cells and mature spermatozoa in each period all reduced in medium-dose group and high-dose group, compared with control group. In high-dose group, the hierarchy of spermatogenic cell in seminiferous tubule basically disappeared, bigger lacuna appeared and interstitium changed to different extent. On 72 day-old, the cross-section of seminiferous tubules deformed, basement membrane became thin accompanied by fracture and damaged extent of interstitium increased in medium-dose group and high-dose group. Abnormal sperms were found in medium dose group. In high-dose group, central blank area in seminiferous tubule was enlarged and mature sperm lacked. The results suggested that medium-dose and high-dose of DHEA had obvious toxic effects on the structure development of testieular tissue in growing mice.
出处
《畜牧与饲料科学》
2013年第12期27-30,共4页
Animal Husbandry and Feed Science
基金
安徽省自然科学基金项目(11040606M88)
安徽科技学院兽医学重点学科基金项目(AKXK20101-2)
关键词
脱氢表雄酮
小鼠
睾丸
毒性作用
dehydroepiandrosterone
mice
testis
toxic effect
