摘要
目的:建立双黄连口服液的HPLC指纹图谱。方法:用Cosmosil C18(4.6mm×250mm,5μm)色谱柱,甲醇-水梯度洗脱;流速:1.0mL/min;柱温:30℃;检测波长330nm。结果:指纹图谱13个共有峰,对其中4个峰进行指认,分别是绿原酸、松脂醇-β-D-葡萄糖苷、连翘酯苷和黄芩苷。结论:该分析方法具有很好的精密度、重现性和稳定性,共有峰的相对保留时间和相对峰面积的RSD均小于3%,符合指纹图谱相关要求,可作为双黄连口服液质量控制的一种有效方法。
Objective：To establish the fingerprints of Shuanghuanglian （SHL） oral liquid by high performance liquid chromatography （HPLC）. Methods： The separation was performed on a Cosmosil C18 column （250mm ×4.6mm,5μm） with methanol - water as the mobile phase at a flow rate of 1. OmL/min. The detective wavelength was set at 330nm and the column temperature was 30℃. Results ：Fingerprints of SHL oral liquid consisted of 13 common peaks. Conclusion： This method is accurate, repeatable and stable. The RSDs of relative retention time and area of aimed peaks which exis- ted in all samples were less than 3 %. The results are in accordance with the request of fingerprinting. The fingerprinting can efficiently control the quality of SHL oral liquid.
出处
《中医药学报》
CAS
2013年第6期24-25,共2页
Acta Chinese Medicine and Pharmacology
基金
黑龙江省新世纪优秀人才支持计划
黑龙江中医药大学青年学术带头人支持计划
关键词
双黄连口服液
指纹图谱
HPLC
Shuanghuanglian oral liquid
Fingerprints
High performance liquid chromatography
