摘要
目的 探讨肝细胞癌组织中端粒酶相对含量与 DNA含量的关系及其临床意义。方法 采用细胞免疫荧光染色技术及流式细胞术共检测 32例肝癌标本 ,同时取无瘤残端作为对照 ,以荧光指数 (FI)表示端粒酶的相对含量。结果 肝癌组织端粒酶相对含量明显高于正常肝组织 (P<0 .0 0 1) ;FI阳性肿瘤中 ,异倍体肿瘤占 85 .7% ,而 FI阴性者无异倍体出现 (P<0 .0 0 2 ) ;端粒酶含量 (FI)与细胞增殖指数 (PI)的相关系数 r=0 .5 4,FI与肝纤维化有关 ,而与肿瘤大小、结节数目、AFP及有无门脉癌栓无关。结论 肝癌组织端粒酶含量增加 ,异倍体发生率高 ,预后差。同步检测肝癌端粒酶和 DNA含量有助于其预后判断。
Objective To invsetigate the relationship between telomerase and DNA content in hepatocleeular carcinoma(HCC).Methods 32 cases of HCC were analysed quantitatively with flowcytometry and cyto-immunofluorescence staining technique,the telomerase content was expressed according to fluorescence index(FI).Results The telomerase content in tumor tissues was significantly higher than that in normal liver tissue(P<0.001).The aneuploid carcinomas were 85.7% in FI-positive carcinoma,while there were no aneuploid carcinoma in FI-negative carcinoma(P<0.002).There is a direct relationship between the telomerase content and proliferative index(r=0.54,P<0.002).The FI was correlated to cirrhosis,but was not significantly correlated to node number,diameter,AFP and portal embolus of the patients.Conclusions The higher content of telomerase and higher incidence of aneuploidwere observed in HCC,It suggested that detection of telomerase and DNA content in tumor be helpful in predicting the outcome of HCC patients.
出处
《肝胆外科杂志》
2000年第6期407-409,共3页
Journal of Hepatobiliary Surgery
关键词
肝细胞癌
端粒酶
DNA含量
预后
Hepatocellular carcinoma Telomerase Deoxyribonucleic acid content
