摘要
目的探讨转化生长因子β1(TGF-β1)和碱性成纤维细胞因子(bFGF)对大鼠口腔黏膜固有层前体细胞(OMLPPC)的增殖及碱性磷酸酶活性的影响。方法体外扩增培养OMLPPC,利用5ng/mL TGF-β1、10ng/ml bFGF单独及联合作用于OMLPPC,利用MTT方法检测OMLPPC的增殖,利用试剂盒检测其碱性磷酸酶活性,以RT-PCR方法检测各组矿化基因骨钙素(OCN)的表达。结果对细胞的增殖,3d时各组间未见显著性差异;7、14d,bFGF单独作用明显促进细胞的增殖(P<0.05),TGF-β1单独作用抑制细胞的增殖(P<0.05)。TGF-β1+bFGF组7d时增殖不受影响,14d时增殖变缓。碱性磷酸酶活性检测,对照组及bFGF组各时间点增加均无显著性差异。TGF-β1组间7、14d比3d时ALP活性有显著性差异(P<0.05)。TGF-β1+bFGF组14d时ALP活性显著增强(P<0.05)。RT-PCR检测OCN结果显示,各组培养14d后,TGF-β1组、TGF-β1+bFGF组表达OCN,对照组、bFGF组未见表达。结论一定浓度的bFGF和TGF-β1联合作用,不仅可保证OMLPPCs的增殖,而且能诱导并促进其向矿化方向的分化。
Objective To examine the effects of TGF-β1 and/or bFGF on the proliferation and the activity of ALPase of rat oral mueosal lamina propria progenitor cell. Methods Rat OMLPPCs were isolated and cultured in vitro. After the OMLPPCs were treated with 5 ng/ml TGF-~I and/or 10 ng/ml bFGF for 3 days, 7 days and 14 days, the growth proliferation activities were observed and analyzed by MTT and the activity of ALPase was examined. The expression of osteocalein （OCN） was examed by RT-PCR. Results There were no significant differences in cell proliferation among all groups after 3 days. ：After 7 days and 14 days, the cell proliferation of bFGF group increased （ P 〈 0. 05 ）, and that of TGF-group decreased （ P 〈 0. 05 ）. After 14 days, the growth proliferation activities of the TGF ＋ bFGF group decreased. There were no significant differences in the activity of Alpase among the groups of bFGF and controls. The activity of Alpase of TGF-131 group was higher in 7 days and 14 days than that in 3 days and in controls（P 〈0. 05）. After 14 days, the activity of Alpase in TGF-β1 ＋ bFGF group increased （ P 〈 O. 05 ）. TGF-β ＋ bFGF group and TGF-β1 group expressed OCN after 14days. Conclusion Besides keeping the proliferation of OMLPPCs,TGF-131 and bFGF can induce them to differentiate with ability of mineralization.
出处
《北京口腔医学》
CAS
2013年第6期305-308,共4页
Beijing Journal of Stomatology
基金
国家自然科学基金(30700946
81271100)
北京市自然科学基金(7112057)
北京市科技新星计划(2011083)
关键词
口腔黏膜固有层
前体细胞
增殖
矿化
Oral mucosa lamina propria
Progenitor cell
Proliferation
Mineralization
