摘要
研究了氨基酸-铜配位化合物的紫外检测反相高效液相色谱分离、测定方法,阐明了色谱分离、检测机理,探讨了流动相pH值、己基磺酸盐、铜离子、缓冲液浓度等对溶质保留和紫外检测灵敏度的影响,确定了最佳色谱分离、检测条件,并用于生物样品分析。
A new reversed-phase liquid chromatographic method with UV-detection for the analysis of amino acid-copper complex without any pretreatment or derivatizatiojn is studied. The mechanism of chromatographic separation and detection is described. The effect of factors such as the concentration of hexanesulfonate, copper ions and buffer as well as pH values is discussed. Guidelines are given for optimization of the separation. The analysis of amino acid samples are examined.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1991年第8期1052-1055,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
氨基酸
铜
络合物
高效液相色谱
Complex of amino acid-copper, Reversed-phase liquid chromatography, UV-detection
