摘要
目的:研究齐墩果酸(Oleanic acid,OA)对人白血病HL-60细胞PI3K、Akt、Cav-1表达的影响。方法:OA作用HL-60细胞后,采用透射电镜观察细胞形态,RT-PCR法检测HL-60细胞PI3K、Akt、Cav-1的mRNA表达。结果:OA作用于HL-60细胞后,细胞形态改变明显,出现凋亡小体,且PI3K、Akt、Cav-1的mRNA表达下调(P<0.01)。结论:OA可抑制HL-60细胞增殖,这可能与其下调HL-60细胞中PI3K、Akt、Cav-1的表达有关。
Objective：To study the effect of oleanolic acid （OA） on the expression of pi3k,akt and cav - 1 genes in leukaem HL -60 cells. Methods：In HL -60 cells, after being treated by OA, the morphological and structural changes of the cells were observed by transmission electro microscopy; the mRNA expressions of pi3k,akt and car - 1 were measured by RT - PCR. Results： After being treated by OA, the HL - 60 cells had obvious mor- phological and structural changes under the ＇transmission electro microscopy, and apoptosis body was observed as well. The mRNA expression of pi3k and akt were down regulated （P 〈 0. 01 ）, the mRNA expression of cav - lwas up regulated （P 〈 0. 01 ）. Conclusion： OA could inhibit the proliferation of HL - 60 cells by down - regulating the expression of PI3 K and Akt , by up - regulating the expression of CAV - 1.
出处
《黑龙江医药科学》
2013年第6期1-2,6,共3页
Heilongjiang Medicine and Pharmacy
基金
国家自然科学基金资助项目
编号:81070428
黑龙江省卫生厅科研课题
编号:2011-438
佳木斯大学科研项目
编号:s2010-102
