摘要
应用随机扩增多态性DNA(RAPD)标记鉴定大白菜杂种商品种子的纯度。用 50个随机引物检测了 2个杂交种北京 57号和北京 10 6号及其亲本 ,引物OPE - 2 0在北京 57号杂交种中产生了有别于双亲的特殊标记 ,引物OPH - 0 6及OPH - 0 7在北京 10 6杂交种中产生了有别于双亲的特殊标记 ,能清楚地区分杂交种及其双亲 ,并将这些引物应用在北京 57号和北京 10 6号杂交种样品的纯度检测中。
Randomly amplified polymorphic DNA(RAPD)markers were applied to distinguish purity of commercial F 1 hybrid seeds of Chinese cabbage.DNA from two F 1 hybrid cultivars,Beijing No 57 and Beijing No 106,and their parental lines were screening with 50 primers.Primer OPE-20 generated in F 1 hybrid Beijing No 57 specific RAPD markers.Primer OPH-06 and OPH-07 generated in F 1 hybrid Beijing No 106 specific RAPD markers.These markers enabled a clear distinction to be made between F 1 hybrids and their parental lines.These primers were used in purity control of hybrid seed production of Beijing No 57 and Beijing No 106 .This result indicated the practical usefulness of RAPD markers in commercial hybrid seeds purity tests of Chinese cabbage.
出处
《华北农学报》
CSCD
北大核心
2000年第4期35-39,共5页
Acta Agriculturae Boreali-Sinica
