摘要
从两个番茄杂种Ｌ４０２、Ｌ４０４及它们的亲本共６份材料的叶片中提取ＤＮＡ，使用国产ＰＣＲ仪，建立了适合番茄ＲＡＰＤ分析的ＰＣＲ条件，进一步在１００个随机引物中找到了５个可用于鉴定这两个杂种纯度的引物，其中ＯＰＫ１４引物可同时用来鉴定两个杂种。利用该法鉴定杂种纯度不仅简单、迅速、可靠，而且因ＤＮＡ的用量少，不影响被检植株的后期生长。
Genomic DNAs were extracted from young leaves of two tomato hybrids and their pa rents. PCR conditions suitable for random amplified polymorphic DNA(RAPD)analysis of tomato were studied.Of 100 arbitrary primers used in the experiment,5 primers amplified polymorphic bands between the Paris of parents. The two hybrids could be distinguished from each other using the primer OPK14.
出处
《园艺学报》
CAS
CSCD
北大核心
1998年第3期247-251,共5页
Acta Horticulturae Sinica
