摘要
目的筛选胎儿宫内发育迟缓大鼠血清中差异表达的蛋白质,寻找与胎儿宫内发育迟缓相关的疾病标志物。方法收集大鼠疾病正常组、IUGR组血浆各3例;TCA法沉淀血清蛋白,建立疾病组和正常组蛋白质的双向凝胶电泳图谱;Image Master分析软件分析差异蛋白质;飞行时间质谱(MALDI-TOF-MS)获得差异蛋白肽指纹图谱,MASCOT引擎搜索蛋白质种类。结果鉴定出个差异蛋白点3个,分别为:IGF-Ⅰ、IGF-Ⅱ、IGFBP-3。结论 IGF-Ⅰ、IGF-Ⅱ、IGFBP-3,可能是胎儿宫内发育迟缓疾病诊断的生物标志物。
Objective Differential serum proteins of rat with Intrauterine Growth Retardation were identifieated with proteomies teehnology ,and associated disease markers of intrauterine growth retardation were found. Method 3 cases rats plasma of normal group and IUGR group were collected, respectively. After serum proteins were extracted with TCA precipitation method ,We established proteins Image of two-dimensional gel electrophoresis of IUGR group and normal grotip. Differential proteins were screened with Image Master software, and peptide mass finger- printing were obtained by MALDI-TOF-MS, then dstabase were searched through the Mascot data engine, and protein identification results were obtained. Results 3 differential protein spots were identificatied. Conclusion IGF- I , IGF- I1,IGFBP-3 are possible bio-markers of In- trauterine Growth Retardation diagnosis.
出处
《世界科技研究与发展》
CSCD
2013年第6期736-738,共3页
World Sci-Tech R&D
基金
重庆市卫生局课题(2009-2-225)资助
