摘要
目的:观察益气化浊胶囊对KKAy小鼠肝脏中胰岛素受体底物1(IRS-1)和2(IRS-2)mRNA和蛋白表达的影响,探讨其增加胰岛素敏感性的作用机制。方法:KKAy小鼠随机分为模型组、益气化浊高剂量组、低剂量组和阳性组,C57BL/6J小鼠作为正常组,每组8只。给药8周后,测空腹血糖(FPG)、血清胰岛素(FINS)及胰岛素抵抗指数(HOMA-IR),采用Real-time PCR技术和Western blot印迹法检测肝脏中IRS-1、IRS-2 mRNA及蛋白表达水平。结果:与模型组比较,高剂量组FPG、FINS、HOMA-IR降低,IRS-1、IRS-2mRNA和蛋白表达水平升高;低剂量组FPG降低,IRS-2蛋白表达明显升高。结论:益气化浊胶囊改善KKAy小鼠胰岛素抵抗,可能与上调肝脏中IRS-1、IRS-2 mRNA和蛋白表达水平有关。
Objective ：To observe effects of Yiqi Huazhuo Capsule on expression of insulin receptor substrate 1 （IRS-1） and IRS-2 mRNA and proteins in liver to explore its possible molecular mechanism in increasing insulin sensitivity. Methods ：KKAy mice were randomly divided into model group,Yiqi Huazhuo Capsule of high dose group, low dose group, positive control group, and C57BL/6J mice were normal control group, which group had 8 mice. 8 weeks after administration, measuring fasting blood glucose （FBG）, fasting insulin （FINS） and calculated insulin resistance index （HOMA-IR）. The expressions of IRS-1 and IRS-2mRNA and proteins were detected with real-time fluorescence quantitative PCR method and Western blot method. Results： As compared with model group, FPG,FINS,HOMA-IR were decreased and mRNA and protein expression levels of the IRS-1 and IRS-2 were increased in high group, FPG was decreased and protein expression levels of IRS-2 was increased in low group. Conclusion：Yiqi Huazhuo Capsule improves FPG and insulin resistance in KKAy mice and its mechanism is probable related to raising expression level of IRS-1 and IRS-2 mRNA and proteins in liver.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2013年第12期1404-1407,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
山西省科技厅攻关项目-益气化浊胶囊治疗2型糖尿病胰岛素抵抗疗效与作用机制研究(20090311055-4)
