摘要
目的:研究黄芪注射液对体外兔心脏再灌注损伤的保护作用。方法:采用体外兔心共生支持系统模型。16只心脏随机分为黄芪组和对照组各8只,缺血期停跳液分别采用黄芪注射液加St.Thomas’液和St.Thomas’液进行心肌保护。常温缺血45 min,再灌注60 min,比较两组再灌注后5 min,30 min和60 min的冠脉回流液中心肌酶含量,组织标本采用HE染色和酸性复红光绿染色评价缺血区面积。结果:再灌注后黄芪组冠脉回流液中心肌酶含量低于对照组,缺血区面积小于对照组,有显著性差异(P<0.05)。结论:缺血过程中停跳液中加入黄芪注射液可以减轻体外心脏再灌注损伤。
Objective： To study the protective effects of Huangqi injection on ischemia reperfusion injury of rabbit heart. Methods： Blood-perfused parabiotic isolated rabbit heart langendorff model was used, Sixteen isolated rabbit hearts were randomly divided into two groups： control groups （n = 8）, Huangqi group （n = 8）. Different composed cardioplegia were used accordingly： St. Thomas＇ injection or St. Thomas＇ injection and Huangqi injection. After a 30-minute stabilization, underwent 45 minutes of global normothermic ischemia, followed by 60 minutes of reperfusion, coronary blood myocardial enzyme leak were measured and compared at 5 minutes, 30 minutes and 60 minutes after reperfusion. Myocardial tissues were stained for observing and comparing ischemia area of the two groups. Results： CK（ereatinin kinase）in Huangqi group was lower than in control group （P〈0.05）, and ischemia area in Huangqi group was smaller than in control group （P 〈 0.05）. Conclusion： Huangqi injection decreases reperfusion injury when used in the eardioplegia during ischemia.
出处
《山东中医药大学学报》
2007年第1期72-74,共3页
Journal of Shandong University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:30572427)
关键词
黄芪
再灌注损伤
心肌保护
Huangqi
reperfusion injury
cardioprotection
