摘要
目的:研究对药远志-石菖蒲对糖尿病大鼠学习记忆功能的干预情况。方法:3月龄雄性Wistar大鼠,随机分为对照组、模型组、远志-石菖蒲干预组(高、中、低剂量组)。采取腹腔注射链脲佐菌素(Streptozotocin,STZ),制备糖尿病大鼠模型。制备对药远志石菖蒲,按成人用量的20,10,5倍的比例分成高、中、低3个剂量组。采用水迷宫实验和气相色谱-质谱联用仪(GC-MS),分别观察糖尿病大鼠的行为学改变和血清甲基乙二醛(MG)变化。采用流式细胞技术,观察糖尿病大鼠海马神经细胞凋亡发生率的变化。结果:Morris水迷宫检测显示,模型组大鼠到达站台时间较对照组明显延长(P<0.05),远志石菖蒲各治疗组所需时间较模型组明显减少(P<0.05);GC-MS测定糖尿病大鼠模型血清MG含量明显升高,约为对照组的2.5倍,远志-石菖蒲治疗组MG含量显著降低(P<0.05);并减少大鼠海马神经细胞凋亡率(P<0.05)。结论:远志石菖蒲能够通过降低大鼠血清MG水平,改善糖尿病大鼠认知障碍。
Objective： To study the improvement of polygala tenuifolia and rhizoma acori tatarinowii in treating cognitive impairment of diabetes mellitus rats. Method： Diabetic rats were established by intraperitoneal injection of Streptozotocin for 3 months. The polygala tenuifolia and rhizoma acori tatarinowii decoction were made. The rats were divided into 5 groups：control group, model group, and polygala tenuifolia and rhizoma acori tatarinowii group （large dose group, medium dose group and small dose group）. observed the change of rat＇s ability of learning and memory function observed were by the way of water maze and examination of the blood serum methylglyoxal through Gas Chromatography-Mass Spectrometry.The percentage of neuron apoptosis was examined through flow cytometry. Result： In the water maze experiment, it was shown that rats in model group needed more time to swim to the platform than the control group, and polygala tenuifolia and rhizoma acori tatarinowii could significantly reduce the swimming time to platform（P〈0.01）. It was shown that the serum methyglyoxal of model group increased about 2.5 times more than the control group, and polygala tenuifolia and rhizoma acori tatarinowii could significantly reduce the serum methylglyoxal and the percentage of neuron apoptosis. Conclusion： Polygala tenuifolia and rhizoma acori tatarinowii could improve diabetes mellitus rat＇s cognitive impairment by reducing serum methylglyoxal.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第24期223-226,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81041022
81102624)
北京市自然科学基金项目(7102073)
北京市科技新星项目(2008A090)
关键词
远志
石菖蒲
糖尿病
认知障碍
甲基乙二醛
Polygala Tenuifolia
Rhizoma Acori Tatarinowii
diabetes mellitus
cognitive impairment
methylglyoxal
