摘要
目的:采用W256癌细胞诱导的骨癌痛模型大鼠探讨槐定碱抗癌痛药效及其作用机制。方法:采用骨髓腔注射W256癌细胞复制骨癌痛大鼠模型;造模10 d后选取模型复制成功的大鼠36只随机分为模型对照组及槐定碱治疗组,另取同期10只大鼠作为正常对照组;治疗组大鼠于造模第15天开始给予槐定碱25 mg·kg-1治疗10 d;各组大鼠给药前、后测量机械痛阈和热痛阈,末次给药后,对大鼠患肢进行放射学和组织病理学观察,并采用免疫组化法检测大鼠患肢肿瘤组织环氧酶-2(COX-2)、血管内皮生长因子(VEGF)的表达。结果:槐定碱可明显提高骨癌痛大鼠机械痛阈和热痛阈值(P<0.05,P<0.01);改善肿瘤引起的骨损伤(P<0.05),明显下调肿瘤组织COX-2,VEGF表达(P<0.05)。结论:槐定碱对W256癌细胞诱导的骨癌痛大鼠有镇痛作用和抑制肿瘤发展作用,其机制可能与下调COX-2,VEGF的表达有关。
Objective： To study the effect of sophoridine against bone cancer pain in bone cancer pain model rats induced by W256 tumor cells and its mechanism. Method ： The rat model of bone cancer pain was reproduced by injecting W256 tumor cells into the rat marrow cavity. Ten days after the model establishment, 36 rats were selected and randomly divided into the model control group and the sophoridine treated group. At the same time, other 10 rats with sham-operation were selected to be the normal control group. Since the 15~h day after the operation, rats in the treated group had been given sophoridine （25 mg~ kg-1 ） for 10 days. The mechani- cal withdrawal threshold and the thermal withdrawal latency of each group were measured before and after the treatment. After the last treatment, the radiological and histopathological observation shall be conducted for sick legs of all rats. The expressions of cyclooxygen- ase-2 （COX-2） and vascular endothelial growth factor （VEGF） in tumor tissues were detected by mmunohistoehemistry. Result： So- phoridine could significantly increase the mechanical withdrawal threshold and the thermal withdrawal latency （ P 〈 0. 05, P 〈 0. 01 ） , significantly relief the bone injury caused by W256 tumor cells （ P 〈 0. 05 ）, and notably down-regulate the COX-2 and VEGF expres- sions in tumor tissues （ P 〈 0.05 ）. Conclusions ： Sophoridine has the effect in relieving pain and inhibiting tumor progression in bone cancer pain rats induced by W256 tumor ceils. Its mechanism may be related to the down-regulated expressions of COX-2 and VEGF.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第23期4134-4137,共4页
China Journal of Chinese Materia Medica
基金
安徽省高等学校省级自然科学研究重点项目(KJ2012Z203)
关键词
槐定碱
W256癌细胞
骨癌痛
COX-2
VEGF
sophoridine
W256 tumor cell
bone cancer pain
cyclooxygenase-2
vascular endothelial growth factor
