摘要
目的 探讨单由庚型肝炎病毒 (HGV)感染对肝脏的致病性。方法 用酶联免疫吸附法检测 36例血清抗 HGV ,用HGV的NS5片段单克隆抗体 (McAb)对 2 0例临床和病理确诊为单一庚型肝炎者行肝组织免疫组化 ,其中急性肝炎 10例 ,慢性肝炎 7例 ,亚急性重型肝炎 1例 ,慢性重型肝炎 2例。结果 ①临床表现 :急性肝炎呈急性起病 ,表现为发热、乏力、恶心、厌油等症状 ,个别有呕吐现象 ;慢性肝炎起病缓慢 ,症状轻 ;重型肝炎呈急性起病 ,有高度乏力、严重消化道症状 ,重者发生肝昏迷。②ALT和AST改变 :急性肝炎和重型肝炎呈中度升高 ,慢性肝炎呈轻度升高 ,重型肝炎可出现“酶胆分离”现象。③肝组织的病理损害 :急性肝炎以肝细胞肿胀和汇管区炎症细胞浸润为主。慢性肝炎以肝细胞肿胀、小叶内碎屑样或灶状坏死、汇管区轻度炎症细胞浸润和 或纤维组织轻度增生为主。重型肝炎以肝细胞亚大块坏死、大量炎症细胞浸润和纤维组织轻度增生为主。结论 HGV感染可呈现急性肝炎。
Objective Our objective is to study the pathogenicity of the single HGV infection. Methods The ELISA was used to check the serum anti HGV antibody of 36 cases.Immunohistochemistry staining of hepatic tissue was conducted by using HGV NS5 McAb in 20 single HGV infected patients who were further confirmed by clinical diagnosis and pathological evidence.These patients included 10 cases of acute hepatitis, 7 cases of chronic hepatitis, 1 cases of subacute severe hepatitis and 2 cases of chronic severe hepatitis. Results (1)Clinical manifestation: Acute hepatitis occurred abruptly, accompanied with fever,inertia,nausea and sometimes with vomiting and oil disgust. Chronic hepatitis developed slowly and presented mild symptoms. Severe hepatitis presented an acute onset,extreme fatigue,serious gastrointestinal symptoms,or even hepatic coma developed. (2)Changes of ALT and AST: ALT and AST increased slightly in chronic hepatitis and moderately in acute and severe hepatitis. The disproportion between ALT and BiL sometimes occurred in severe hepatitis. (3)Pathological changes in hepatic tissue: Acute hepatitis was characterized by hepatocyte swelling and inflammatory cells infiltratd in portal duct areas. Chronic hepatitis was identified by hepatocyte swelling, piecemeal necrosis or spotty necrosis,slight infiltration of inflammatory cell in portal duct areas and fibrosis. Severe hepatitis appeared as hepatic cell necrosis,inflammatory cell infiltration and slight fibrosis. Conclusion Infection of HGV can give rise to acute hepatitis,chronic hepatitis and severe hepatitis.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2000年第4期361-363,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
肝炎
庚型
肝
病理学
免疫组织化学
Hepstitis G
Liver/Pathology
Immunohistochemistry
