摘要
目的:分析我国华南地区庚型肝炎病毒(HGV)5′端非翻译区(5′UTR)基因序列及非甲一戊型肝炎病人中 HGV感染情况。方法:在 HGV 5′UTR 设计引物,采用逆转录一套式聚合酶链反应(RT-PCR)检测 HGV RNA,对扩增产物进行分子克隆,以荧光法(Applied Biosystems)测序。结果:63例非甲—戊型肝炎病人中6例 HGV RNA 阳性(9.5%)。对其中一株输血后 HGV(CE-S)5′UTR 核苷酸序列分析与 Linnen 等报道的2株(PNF2161,R10291)和 Leary 等报道的GBV-C 相比较,其核苷酸序列的同源性分别为89.2%,88.7%与87.1%。结论:本研究证实我国华南地区存在 HGV 感染。HGV 高度保守区5′UTR 的分子克隆与核昔酸序列的测定对开展 HGV 感染的基因诊断、流行病学调查等均具有重要意义。
Objective:To analyse partial nucleotide sequence of hepatitis G virus(HGV)isolated from a patient with post-transfusion non A-E hepatitis in Guangdong.Methods:A reverse transcription polymer- ase chain reaction(RT-PCR)assay with nested primers from the 5'untranslated region(5'UTR) of HGV genome was established to detect HGV RNA and the applied biosystems for nucleotide sequening of IIGV cDNA were used.Results:6 of 63 patients with non A-E hepatitis were positive for HGV RNA(9.5%). Partial gene(5'UTR)of a Ctfinese isolate(Ch-S)of IIGV was cloned,sequenced and compared with some published sequences of HGV strain PNF 2161,R10291 and GBV-C reported by Linnen,Zhang and Leary, respectively.The nucleotide homology were 89.2% between HGV Ch-S and PNF 2161,88.7% between Ch- S and R10291,87.1% between Ch-S and GBV-C.Conclusion:The results of this study confirm the epidemic of HGV infection in Southern China.The sequencing of highly conserved 5'UTR of the Chinese HGV strain has important implication for the development of genetic diagnostic assays for HGV infection.
出处
《中华肝脏病杂志》
CAS
CSCD
1997年第4期227-228,共2页
Chinese Journal of Hepatology
关键词
庚型肝炎病毒
基因
聚合酶链反应
分子克隆
Hepatitis G virus
Gene
Polymerase chain reaction
Molecular clone
Sequence analysis
