摘要
目的与方法庚型肝炎病毒(HGV)基因组,为正链单股 RNA 病毒,全长约9400核苷酸左右。根椐5′端非编码区基因序列设计合成巢式 PCR 两对引物,逆转录-巢式 PCR 扩增产物,经低融点琼脂糖凝胶电泳和柱层析纯化,获得高纯度 cDNA 扩增片段,用(长臂)光敏生物索标记上述 cDNA 片段,制备成 DNA 生物素探针。通过 Southern 杂交检测转录后第一次 PCR 扩增较大的 DNA 片段。结果证实 PCR 扩增的 HGV RNA 血清,为以后 HGV 分子病毒学研究提供生物材料和证据。结论 Southern 杂交结果从基因诊断的分子水平,证实我院临床患者中存在着庚型肝炎病毒感染。
Objective To identify infection of HGV in patients with hepatitis.Methods The primes were designed and synthesized based on the 5′end sequence of hepatitis G virus(HGV).The products from the first round of PCR were purified from low-melting agarose and resine and the biotin labeled probes for targeting the specific PCR products of HGV RNA were prepared.Results The 4 cases of positive sera were detected by Southern blotting after the first round of PCR.Conclusion The results showed that the HGV infection existed in the Chinese.
出处
《中华肝脏病杂志》
CAS
CSCD
1997年第1期12-13,共2页
Chinese Journal of Hepatology
