摘要
目的 探讨乙型肝炎患者的乙型肝炎病毒 (HBV)血清学标志 (HBVM )与HBVDNA检测结果的相关性与临床意义。方法 对 414例乙型肝炎的HBVM和HBVDNA检测结果进行比较。HBVM用ELISA定量分析法检测 ,HBVDNA用斑点杂交法检测。结果 急性、慢性乙型肝炎患者中HBVDNA的阳性率与乙型肝炎肝硬化患者的HBVDNA阳性率比较 ,差异有显著性 ;HBsAg、抗 HBe、抗 HBc阳性和HBsAg、HBeAg、抗 HBc阳性组的HBVDNA阳性率比较 ,差异无显著性 ;HBsAg和 或HBeAg的滴度与HBVDNA阳性率呈正相关关系。 结论 HBVDNA是评价HBV活动最理想的标志 ;抗 HBe的出现不能作为HBV复制停止的指标 ;
Objective Serum samples of 414 cases with hepatitis B were detected for hepatitis B markers (HBVM) and HBV DNA,in order to investigation the relationship between HBV M and HBV DNA,and its clinical significant. Methods HBV M and HBV DNA were detected by ELISA and blot hybridization methods respectively.Results HBV DNA positive rates in the groups of acute and chronic hepatitis B were higher than that in the group of liver cirrhosis ( P < 0.01 ); HBV DNA positive rates had no apparent difference between the HBsAg,HBeAg, anti HBc positive group and the HBsAg, anti HBe,anti HBc positive group. Direct relativity was shown between the titer of HBsAg and/or HBeAg and the positive rate of HBV DNA. Conclusion HBV DNA can be treated as a sign of HBV activity; the appearance of anti HBe can not be taken as indicator of ceasing of HBV replication; the changes in HBsAg and HBeAg titers may be used as clinical evaluation on HBV replication and index for anti viral therapeutic effect.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2000年第4期352-354,共3页
Chinese Journal of Experimental and Clinical Virology
