摘要
目的探讨不同剂量的细菌内毒素对WHBE兔致热反应的敏感性。方法静脉注射不同剂量(1.25、2.5、5、10 EU/kg)细菌内毒素诱发WHBE兔和日本大耳白兔发热反应,并设空白对照组,记录升温曲线图,计算发热峰值、180 min时体温差、发热指数以及升温率,并通过曲线拟合计算出体温升高0.6℃时的发热阈值。结果随细菌内毒素注射剂量的增加致WHBE兔和日本大耳白兔的发热反应增强,性别因素对细菌内毒素所致WHBE兔和日本大耳白兔发热反应无明显影响(P>0.05)。5 EU/kg和10 EU/kg的细菌内毒素所致WHBE兔的致热反应均高于日本大耳白兔,且WHBE兔的升温率分别为60.7%和92.9%,日本大耳白兔的升温率分别为39.3%和82.1%。另外,WHBE兔和日本大耳白兔的发热阈值分别为5.72 EU/kg和7.93 EU/kg。结论细菌内毒素对WHBE兔具有较好的致热敏感性,适于致热原物质的检查。
Objective To explore the sensitivity response to bacterial endotoxin of WHBE rabbits. Methods The fever was studied after the WHBE rabbits and Japanese white rabbits were intravenously injected bacterial endotoxin in different doses （1.25, 2.5, 5, 10 EU/kg） , and on the normal rabbits as the control group. Heating curves were drew, and analyzed the fever peak, the temperature difference at 180min, temperature response index and heating rate. Through curve fitting the fever threshold doses were calculated. Results The results of the study showed that the increase of bacterial endotoxin dosage had a promotive effect on the febrile response of the rabbits. It had no significant influence on gender factor for the febrile response of the WHBE rabbits and Japanese white rabbits induced by bacterial endotoxin. 5 EU/kg and 10 EU/kg dosages of bacterial endotoxin-induced pyrogenic reactions on WHBE rabbits were higher than those of Japanese white rabbits, and the heating rates of WHBE rabbits were 60. 7% and 92.9% , while the heating rates of Japanese white rabbits were 39.3% and 82. 1%. Furthermore, the pyrogenic threshold doses of endotoxin in WHBE rabbits and Japanese white rabbits were 5.72 EU/kg and 7.93 EU/kg after intravenous injection. Conclusion WHBE rabbits bad better sensitivity on pyrogenic reactions induced by bacterial endotoxin and are suitable to pyrogen test.
出处
《中国比较医学杂志》
CAS
2013年第11期13-17,共5页
Chinese Journal of Comparative Medicine
基金
浙江省教育厅一般项目(Y201121693)
"浙江省卫生高层次创新人才培养工程项目"
关键词
WHBE兔
细菌内毒素
热原
敏感性
WHBE rabbit
Bacterial endotoxin
Pyrogen test
Sensitivity
