摘要
利用Hungate严格厌氧技术从窖齡为20年的老熟窖泥中分离到1株菌株S11-18-8。通过16S rRNA序列比对,其与NCBI中的Clostridium mesophilum strain SW408(NR_044386)的同源性最高,高达99%。研究了该菌株在乳酸钠培养基、葡萄糖培养基、黄水培养基和葡萄糖-糟醅浸提液培养基等不同发酵条件下的发酵特性。结果表明,该菌株在各发酵条件下均能够产生H2、乙酸和丁酸。在黄水培养基中细菌S11-18-8不仅能够发酵代谢产生丁酸和乙酸等有机酸,还能起到很好的"降乳"作用。在葡萄糖糟醅培养基中主要也是代谢产生乙酸和丁酸,同时起到"增己"的作用。因此,推测该菌株在白酒发酵过程有着重要作用。
A H2-producing, anaerobic bacterial strain S 11-18-8 was isolated from aged pit mud （pit age of 20 years） by Hungate anaerobic technol- ogy. Phylogenetic analysis based on 16S rRNA gene sequencing identified strain S11-18-8 as a species of Clostridium with 99.0 % 16S rRNA gene sequence similarity with Clostridium mesophilum strain SW408 （NR 044386）. In this study, the fermenting characteristics of S11-18-8 in sodium lactate medium, glucose medium, yellow water medium and glucose-fermented grain leach liquor medium were investigated. The results showed that strain S11-18-8 could produce H2,acetic acid and butyric acid in all medium, besides, S11-18-8 could reduce lactic acid significantly in yellow water medium and increase hexanoic acid in glucose-fermented grain leach liquor medium. Accordingly, it was believed that such strain played important roles in liquor fermentation.
出处
《酿酒科技》
北大核心
2013年第12期21-24,共4页
Liquor-Making Science & Technology
基金
国家自然科学基金资助项目(31071585)
关键词
产氢细菌
分离
代谢特性分析
H：-producing bacterial strain
isolation
metabolic characteristics analysis
