摘要
为了探讨中国传统白酒窖池糟醅中的原核微生物区系的构成及变化,运用PCR扩增技术和16SrDNA序列同源性分析等方法测定糟醅中原核微生物的16SrDNA基因全序列,根据与基因数据库中相似菌群16SrDNA序列的同源性,分析窖池糟醅中原核微生物的生理特性和区系分布。结果发现,发酵60d的浓香型白酒糟醅中,真细菌主要分为6个菌群:低G+Cmol%革兰氏阳性菌、高G+Cmol%革兰氏阳性放线菌群、革兰氏阴性拟杆菌群、革兰氏阴性变形杆菌群、革兰氏阳性纤毛菌群和TM7门,其中β-变形杆菌群约占窖池糟醅中原核微生物的80%;古菌主要为产甲烷古细菌,包括Methanoculleus群和Methanospirillum群。通过不同窖池的比较分析得出,微生物的多样性与窖池微生态密切相关。
In order to know the compositions and changes of pronucleus microbial floras in the fermented grains in the pits for Luzhou-flavor liquor, the techniques including PCR technique and 16S rDNA sequence homology analysis etc. were used to measure 16S rDNA gene total order of pronucleus microbes, then the physiological characteristics and flora distribution of pronucleus microbes were analysed according to their homologies with similar flora 16S rDNA order in Genebank. And the results indicated that eubacteria in Luzhou-flavor fermented grains by 60 d fermentation were mainly divided into 6 microbial floras as follows;low G+C mol % Gram-positive bacteria,high G+C mol % Gram-positive actinomycete,Gram-negative bacteroid,Gram-negative bacillus proteus,Gram-positive cilia bacteria and TM7 flora. Among all the microbial floras,β-bacillus proteus made up approximately 80 % of the total pronucleus microbes and archaebacteria mainly presented as methane-producing archaebacteria including Methanoculleus flora and Methanospirillum flora. Besides, it was concluded through the analysis and comparison of different pits that the diversity of microbes was closely related to pits microecology.
出处
《酿酒科技》
北大核心
2005年第7期22-25,共4页
Liquor-Making Science & Technology
基金
四川省经贸委重点项目(编号:川经贸技术2002-236)
