摘要
该研究采用家兔内毒素血症模型 ,探讨磷脂酶A2 (PLA2 )抑制剂磷酸氯喹 (CQ)对内毒素血症时细胞、亚细胞膜的保护效应。实验结果表明 ,注射内毒素后MAP显著下降 ,8h存活率为 5 7% ;血浆、红细胞膜、肝线粒体膜PLA2 活性及MDA含量显著升高 ,并随着伤情的加重而升高。而CQ预处理内毒素组注射内毒素后MAP下降幅度显著小于内毒素组 (P <0 .0 5 ) ,血浆、红细胞膜、肝线粒体膜PLA2 活性及MDA含量也显著低于内毒素组 ,动物 8h活存率达 87.5 %。PLA2 抑制剂CQ能大大提高动物活存率和活存时间 ,改善血液动力学 ,抑制PLA2 活性 ,保护细胞膜。提示
Protective effects of phospholipase A 2(PLA 2)inhibitor chloroquine(CQ)on cell membrane in endotoxemia was investigated with rabbits endotoxemia model in this study.The experimental results showed that mean arterial blood pressure (MAP) decreased significantly and survival rate in 8h was 57% after injection of endotoxin;PLA 2 activity and malondialdehyde(MDA)content in plasma,red cell membrane and hepatocellular mitochondria were increased markedly and increased with the development of the injured degree,There was no signifinant lower MAP in the pretreated group compared with endotoxin group( P <0.05).PLA2 activity and MDA content in the pretreated group by CQ were elevated compared with endotoxin group,animal 8h survival rate was 57% in endotoxin group and 87.5% in CQ pretreated group.PLA2 inhibitor CQ can evidently improve hemodynamic index,inhibited PLA2 activity,protected cell membrane,prolonged animal survival time and enhanced survival rate.According to these results,CQ is very effective on against shock and SIRS
出处
《中国现代医学杂志》
CAS
CSCD
2000年第12期5-7,共3页
China Journal of Modern Medicine
