摘要
目的:观察运动、膳食干预下肥胖大鼠肝脏细胞信号抑制因子3(SOCS3)、长型瘦素受体(LP-Rb)蛋白水平的变化,探讨SOCS3在肥胖大鼠外周瘦素抵抗发生及改善过程的作用。方法:以高脂饮食制备肥胖瘦素抵抗大鼠动物模型,随机分组为高脂膳食对照组(H16)、高脂膳食运动组(HHE)、普通膳食运动组(HNE)、普通膳食组(HN)及原有的空白对照组(N16)。运动、膳食干预8周后,采用Western blot法检测大鼠肝脏中SOCS3、LP-Rb水平,放射免疫法测定血清胰岛素水平,用酶联接免疫吸附法测定血清瘦素水平。结果:1)8周干预措施后,各组血清瘦素水平仍显著性高于与N16组(P<0.05),但HNE、HHE组已显著性低于H16组(P<0.05),且HNE显著性低于HHE及HN组(P<0.01);2)运动、膳食干预并未造成各干预组大鼠肝脏LP-Rb蛋白水平与H16组的显著性差异(P>0.05)3)H16组与N16组大鼠肝脏SOCS3蛋白水平无显著性差异,只有HNE组大鼠肝脏SOCS3蛋白较H16组及N16组有显著性下降(P<0.01),且显著性低与HHE及HN组(P<0.01)。结论:1)高脂膳食导致大鼠肝脏LP-Rb蛋白水平显著下降可能是外周瘦素抵抗的发生机制之一。但运动、膳食干预并不能提高大鼠肝脏LP-Rb蛋白水平,提示肥胖大鼠瘦素抵抗的改善可能与肝脏LP-Rb蛋白水平无关。2)运动联合膳食干预可以显著性下调大鼠肝脏SOCS3蛋白水平,但却对LP-Rb无任何影响作用,提示SOCS3可能是通过影响瘦素受体后信号水平调节体内物质能量代谢,而改善外周瘦素抵抗状态。
Objective：To observe the changes of SOCS3, LP - Rb in liver of obesity leptin resistance rats through exercise and diet interventions in order to study the effect of SOCS3 on peripheral leptin resistance. Methods ： Healthy male SD rats were fed with high - fat diet to set up the animal model of leptin resistance and dietary obesity. The model group rats （ n = 40） wererandomized into four groups： high fat diet group （H16）, high fat diet and exercise group （HHE）, common diet and exercise group （HNE） , common diet group （HN）. The control group was recorded as N16. Each group rats were given exercise or di- et interventions for 8 weeks. SOCS3, LP - Rb in liver was observed by Western blot. The serum insulin was measured by radio immunity. The serum leptin was measured by ELISA. Results： 1 ） After 8 -weeks intervention, serum leptin of every group were significantly higher than that of N16 （ P 〈 0.05）. Leptin of HNE, HHE groups were lower than that of H16 （ P 〈 0.05 ）, and leptin of HNE group was significantly lower than those of HHE, HN groups （ P 〈 0.01 ）. 2） LP - Rb of each intervention group had no difference with level of H16. 3 ） SOCS3 in liver had no difference between H16 group and N16 group. SOCS3 of HNE group in liver was significantly lower than that of H16, N16 groups （ P 〈 0.01 ）, furthermore, it was lower than that of HHE, HN groups （P 〈 0.01 ）. Conclusions ： 1 ） Lowering of LP - Rb in liver induced by high fat diet was assosiated with pe- riph leptin resistance. Exercise combination with diet invention cant improve level of LP- Rb in liver, so amelioration of obe- sity leptin resistance rats has noting to do with LP - Rb in liver. 2） Exercise combination with diet invention can significantly degrade protein level of SOCS3 in haper, but no effection on LP - Rb. SOCS3 possibly improved peripheral leptin resistance through effecting signal of post - LP - Rb.
出处
《广州体育学院学报》
CSSCI
北大核心
2013年第6期90-96,共7页
Journal of Guangzhou Sport University
基金
南京邮电大学人才引进科研启动基金(NY210086)
