摘要
目的用高脂高热量饲料配方建立营养性肥胖大鼠动物模型,观察营养性肥胖对雄性大鼠睾丸发育的影响。方法用改进的高脂、高热量饲料喂养3周龄SD雄性大鼠6周,观察大鼠体重、睾丸重量、光镜下睾丸组织结构改变,以及血中睾酮(T)和雌二醇(E2)水平,并与对照组进行比较。结果喂养3周后,实验组大鼠平均体重较对照组超重29%,6周后超重30%。光学显微镜下可见实验组大鼠睾丸大部分曲精小管发育欠佳,管腔中4层细胞稀疏,层次紊乱;实验组E2明显高于对照组,TE2明显低于对照组(P<0.05)。结论高脂、高热量饮食可以引起大鼠营养性肥胖;营养性肥胖影响了大鼠的睾丸发育,可能和血中雄性激素水平相对降低有关。
Objective\ We established diet-induced obesity animal model and observed the influence of obesity on testicle of the male rats. Methods Rats in model group were fed with fat-enriched diet for 6 weeks. We measured the body weight and the testis weight and observed the development of testicle by microscope, and compared with those of control group. Meanwhile, we measured the serum levels of testosterone^T) and estradiol ~E2). Results The average body weight in model group rats was higher 29% than that in control group at 6 weeks old, 30% at 9 weeks old. We observed under microscope that most of the convoluted seminiferous tubule developed no good in model group, the 4 layer cells was sparse and the arrangement of them was not in order. The testosterone to estradiol ratios^T/E2) in model group was significant lower then that of control group^P<0.05). Conclusion The fat-enriched fat diet can induce obesity. It influenced the development of the testicle. This probably related to decrease of the testosterone to estradiol ratios.
出处
《卫生研究》
CAS
CSCD
北大核心
2005年第4期477-479,共3页
Journal of Hygiene Research
关键词
营养性肥胖
睾丸
大鼠
diet-induced obesity, testicle, rat
