摘要
目的采用一测多评方法,建立大败毒胶囊中总蒽醌的含量测定方法。方法采用C_(18)柱(4.6mm×250mm,5μm),以甲醇-0.1%磷酸溶液(85:15)为流动相,流速1.0 mL·min^(-1),柱温25℃,检测波长254 nm,建立大黄酸、大黄酚和大黄素甲醚与内标物大黄素间的相对校正因子,并以相对保留时间作为目标峰的定位标准。结果色谱峰分离度良好,大黄酸在5.724×10^(-2)~0.572 4μg、大黄素在4.58 4×10^(-2)-0.458 4μg、大黄酚在6.368×10^(-2)~0.636 8μg、大黄素甲醚在6.548×10^(-2)-0.654 8μg内呈良好线性关系,r(0.999 7~0.999 9),加样回收率大黄酸为(97.8±1.4)%,大黄素为(100.7±1.0)%,大黄酚为(98.6±0.6)%,大黄素甲醚为(97.0±1.4)%。大黄素对大黄酸、大黄酚和大黄素甲醚的相对校正因子分别为1.19,1.40,1.06。结论该方法简单、快速。准确、可靠,可用于大败毒胶囊中蒽醌类成分的质量控制。
OBJECTIVE To establish a auantitative analysis of multi-components by single marker（QAMS） method for determi- ning the content of anthraquinones in Dabaidu capsules（ traditional Chinese medicines）. METHODS The relative correction factors （RCFs） of emodin to rheiM, chrysophanol, and physcion were determined on a Cls column （4. 6 mm x 250 mm, 5~m） with methanol- 0. 1% phosphoric acid （85： 15） as mobile phase. The flow rate was 1.0 mL ~ min-t. The column temperature was set at 25 ~C. The detection wavelength was set at 254 nm. The relative retention time （RRT） was used to define the target peak. RESULTS The cali- bration curves for rheiM, emodin, chrysophanol, and physcion showed good linearity in the ranges of 5. 724 ± 10-2 - 0. 572 4 μg, 4. 584 × 10 -2 _ 0. 458 4μg, 6. 368× 10 -2 _ 0. 636 8μg and 6. 548 × 10 2 -0. 654 8 μg, r（0. 999 7 -0. 999 9）, respectively. The recov- eries were （97.8~1.4）%, （100.7±1.0）%, （98.6±0.6）% and （97.0±1.4）%, respectively. The RCFs were 1.19, 1.40 and 1.06. CONCLUSION The method is simple, rapid, accurate and reliable. It can be applied to control the quality of anthraquinones in Dabaidu capsules.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2013年第23期2039-2044,共6页
Chinese Pharmaceutical Journal
关键词
一测多评
相对校正因子
大败毒胶囊
蒽醌
高效液相色谱法
The method is simple, rapid, accurate and reliable. It can be applied to control the quality of anthraquinones in Dabaidu capsules.
