摘要
目的建立大败毒胶囊的鉴别和含量测定方法。方法采用TLC法及HPLC法。结果TLC鉴别陈皮、赤芍、白芷等药材,色谱清晰,分离度好。HPLC测定大黄素、大黄酚,进样量与峰面积的线性关系良好(分别为0.9994、0.9992)。平均回收率分别为95.10%(RSD=1.76%)、94.5%(RSD=1.81%)。结论鉴别重复性好,专属性强;含量测定方法灵敏、准确。
OBJECTIVE To establish an identification and a determination method for Dabaidu capsule. METHODS Both TLC and HPLC method were used. RESULTS TLC method could indentify Pericarpium citri reticulatae, Radix paeoniae rubra and Radix Angelicae dahuricae, while HPLC method could quantitatively determine emodin and chrysophanol contents in Dabaidu capsule. The linear range for emodin was 0.01 - 0.08μg（ r=0.9994）, the average recovery was 95.1% with RSD of 1.76%. The linear range for chrysophanol was 0.02 - 0.17 μg （ r = 0.9992）, the average recovery was 94.5 % with RSD of 1.81%. CONCLUSION The indentification methods are reproducible and specific, the quantitative determination method is sensitive and accurate.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2006年第1期96-98,共3页
West China Journal of Pharmaceutical Sciences
