摘要
为探讨内毒素(ET)对大鼠肝脏c-myc基因的表达影响及阳离子(CA)对该过程的保护效应,本试验将72只清洁级SD大鼠随机分为对照组、内毒素组和阳离子A保护组,3组经相应处理后分别在3、4、8、12h采集肝脏作为样本。采用实时荧光定量RT-PCR检测法和免疫组化法分析,结果显示:在mRNA水平和蛋白水平上,ET可上调cmyc基因的表达,从而促进肝细胞凋亡,最终导致肝细胞损伤,CA则能明显下调c-myc基因的表达,从而抑制肝细胞凋亡,对ET诱导的肝损伤具有明显的保护效应。
To disscuss the effect of endotoxin on expressions of c-myc and the protective effect of Cation A on the liver,seventy-two SD rats were randomly divided into the normal control group, ET treatment group and Cation A（CA） and ET treatment group. Rats of three groups were killed to collect liver tissue for real-time PCR and immunohistochemical analysis. The result indicated that the change tendency of c-myc gene was the same in mRNA level and protein level, ET could up-regulate expression level of the c-myc in the liver, which induced the apoptosis of hepatocyte. On the contrary,CA have significant protective effect on injuries of ET to hepatocyte.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第12期1865-1868,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金(30560113
31260594)
云南省高校科技创新团队支持计划资助项目(云教科[2011]14号)
云南省现代农业生猪产业技术体系建设(云财农[2009]171号)
