摘要
利用高效液相色谱法分别测定正常对照组(Ⅰ组)、内毒素组(Ⅱ组)和内毒素+阳离子A拮抗组(Ⅲ组)中兔红细胞膜磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、磷脂酰丝氨酸(PS)和磷脂酰肌醇(P I)含量。结果显示,Ⅱ组中除PE在处理后0.5 h没有明显变化外(P>0.05),PC、PE、PS、P I含量在0.5、1、3、5、7 h均低于Ⅰ组(P<0.05,P<0.01);而Ⅲ组中除PS、PE和P I在处理后0.5 h、PC在1 h、P I在3 h没有明显变化外(P>0.05),整个时间段中上述4种磷脂的含量均高于Ⅱ组(P<0.05,P<0.01)。可见,内毒素能使兔红细胞膜磷脂含量降低,而阳离子A在一定程度上具有对内毒素的拮抗作用和对红细胞膜的保护效应。
To investigate the effect of endotoxin on phospholipid metabolism of erythrocyte membrane （ECM） and cation A antagonism in rabbits, HPLC was used to measure the contents of phosphatidylcholine （PC）, phosphatidylethanolamin （PE）, phosphatidylserine （PS） and phosphatidylinositol（PI） in erythrocyte membrane of three groups ：the normal controls （group Ⅰ ）, endotoxin treatment group（group Ⅱ） and cation A and endotoxin treatment group（group Ⅲ ）. It was found that the contents of the four phospholipids in groupⅡ were lower than those of group Ⅰ during experiment（P〈0.05,P〈0. 01） except the contents of PE were no significant difference ：as compared to group Ⅰ at the 0. 5 h（P〈0.05）. Except no significant difference in PS,PE and PI at the 0.5 h,PC at the 1 h and PI at the 3 h（P〈0.05）,the significantly increase in the contents of four phospholipids in group Ⅲ were observed as compared to group Ⅱ （P〈0. 05,P〈0. 01）. These results indicated that endotoxin could decrease the major membrane phospholipids in ECM ,whereas CA possessed a prominent protective effect for ECM to antagonize the endotoxin injures and provide a valuable evidence for the use of some antagonistic endotoxin drugs.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第4期397-400,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30360079)
关键词
内毒素
阳离子A
红细胞膜
磷脂
endotoxin
cation A
erythrocyte membrane
phospholipid
