摘要
目的为了探明癌基因ｃ－ｅｒｂＢ１、ｃ－ｍｙｃ，抑癌基因ｐ５３和ｐ１６在胶质瘤发生发展中的作用，以及上述基因蛋白检测对胶质瘤诊断、分级的临床意义。方法用免疫组化ＡＢＣ法检测４种癌基因蛋白产物在６５例人脑胶质瘤中的表达，并分析与胶质瘤病理分级间的关系。结果ｐ５３、ｃ－ｅｒｂＢ１、ｃ－ｍｙｃ过度表达率随胶质瘤级别升高而逐渐升高，前两者在胶质瘤良性组和高度恶性组间差异有显著性（Ｐ＜０．０５）；ｐ１６在高度恶性组表达缺失率高于其它组，但差异不显著；４种癌基因、抑癌基因在胶质瘤中有协同表达。结论上述４种基因在胶质瘤的形成和发展中发挥着不同程度的作用，对ｐ５３、ｃ－ｅｒｂＢ１基因蛋白的检测或多种癌基因蛋白联合检测，对胶质瘤的病理分级和预后判断有积极的意义。
Objective To realize the significance of cerbB1 and cmyc protooncogenes, p53 and p16 tumor suppressor genes in the development and pathologic diagnosis of human glioma. Methods The expression of p53, cerbB1, cmyc and p16 gene proteins was detected immunohistochemically in 65 cases of glioma. The relationship between these results and the pathological grades was determined. Results The overexpression of mutant p53, cerbB1 and cmyc was in accordance with the increasing grade of glioma malignancy, showing significant difference (P<0.05) between the benign group and highly malignant group in p53 and cerbB1, while the deletion of p16 protein was more frequent in the highly malignant group than in any other group. These 4 genes showed coexpression in some of the gliomas. Conclusions The 4 genes, especially p53 and cerbB1, play an important role in the development of glioma; the detection of these gene proteins has a positive significance on malignancy determination for glioma.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1997年第6期343-345,共3页
Chinese Journal of Pathology
关键词
神经胶质瘤
P53蛋白
免疫组织化学
Glioma Protein p53 Protooncogene proteins cmyc Immunohistochemistry
