摘要
受无糖培养技术启示，在太子参组培苗育苗过程中，以液体培养基取代固体培养基，应用纸桥法，以太子参幼苗茎段为外植体，经有效诱导芽分化后转入以蛭石为基质的增殖培养基增殖培养，并诱导生根，成苗后辅以自然光照，通过培养基改进对太子参组培苗质量进行研究。结果表明：茎段在合MS＋0．1mg／LNAA＋0．5～1．0mg／L6-BA＋2％蔗糖的液体培养基滤纸桥上的芽分化率达80％以上。不定芽在以蛭石为基质的MS＋0．1mg／LNAA＋1mg／L6．BA十2％蔗糖的培养基中增殖并生根，生根后的试管苗转到低糖（0．5％）或无糖培养基中，经自然光照培养能得到高质量的试管苗。
Inspiraed by sugar-free culture technique, liquid culture medium replacement for solid culture medi- um, the stems of pseudostellaria heterophylla have been used as explants which incubate on filter paper bridg- es in test tubes containing liquid basal medium to study quality in tissue-cultured shoots. The results show that MS＋0. lmg/L NAA＋0.5 ~ 1.0mg/L 6-BA＋2% sucrose was liquid basal medium for filter paper bridges, and the shoot differentiation rate was up to 80%. The adventitous sprouts are propagated and rooted in ver- miculite medium of MS＋0. 1 mg/L NAA＋ 1mg/L 6-BA＋2% sucrose. Then high quality test-tube plantlets were cultivated in low sugar （0.5%） or sugar-free culture medium by natural lighting.
出处
《六盘水师范学院学报》
2013年第4期24-26,共3页
Journal of Liupanshui Normal University
基金
六盘水师范学院重点学科建设(No.:52020-2012-05-01-02)
关键词
太子参
液体培养
组培苗
质量
pseudostellaria heterophylla
liquid culture
tissue culture seedling
quality
