摘要
以太子参〔Pseudostellaria heterophylla(M iq.)Pax〕二倍体组培苗为实验材料,运用正交实验设计和组织培养方法,对太子参试管苗快速繁殖技术进行优化,并进行了同源四倍体的诱导与鉴定。结果表明,太子参最佳的繁殖培养基为含1.0 mg.L-16-BA和0.2 mg.L-1NAA的MS培养基;最佳生根培养基为含0.1 mg.L-1IAA、0.2mg.L-1NAA和1.0 mg.L-1ABT或0.2 mg.L-1IAA、0.2 mg.L-1NAA和1.0 mg.L-1ABT的1/2MS培养基。诱导同源四倍体的最佳处理方法为:用0.2%秋水仙素处理22 h或用0.3%秋水仙素处理12 h,所诱导的同源泉四倍体染色体数目为2n=4x=64条。
The method of rapid propagation in tissue culture of Pseudostellaria heterophylla （ Miq. ） Pax was optimized by using orthogonal experiment. The autotetraploid induction and identification were carried out in vitro. The results indicated that the best multiplying medium was MS containing 1.0 mg · L^-1 6-BA and 0.2 mg · L^-1 NAA. The strong root was induced on the 1/2 MS medium containing 0.2 mg · L^-1 NAA, 1.0 mg · L^-1 ABT and 0.1 or 0.2 mg · L^-1 IAA. The autotetraploid strains could be induced by immersing buds in 0.2% colchicine solution for 22 h or in 0.3% colchicine solution for 12 h. The chromosome number of autotetraploid strain was 2n =4x =64.
出处
《植物资源与环境学报》
CAS
CSCD
2006年第2期50-54,共5页
Journal of Plant Resources and Environment
关键词
太子参
快繁
多倍体
诱导
鉴定
Pseudostellaria heterophylla （Miq.） Pax
rapid propagation
autotetraploid
inducing
identification
