摘要
以蓖麻品种淄5号和秀蓖2号的成熟种子胚轴为外植体,在含0.25 mg/L苯基噻二唑基脲(TDZ)的改良MS(蔗糖20 g/L、琼脂9 g/L)固体培养基上诱导生芽。结果显示:淄5号和秀蓖2号平均每个外植体可产生4个不定芽,2品种间产生不定芽的数量没有明显差异;不定芽经伸长、诱导生根能发育成完整的植株,2品种在根分化能力上没有明显差异。综合分析,这2个蓖麻品种基因型对体外再生体系的建立没有明显影响。
The hypocotyl of Zi No.5 and Xiubi No.2 mature seeds were used as explants. Adventitious buds were induced on optimized MS medium（20 g/L sucrose, 9 g/L agar） with 0.25 rng/L TDZ. The results showed that an average of 4.0 normal buds was produced on each explant from Zi No.5 and Xiubi No.2 and the difference in the quantities of normal buds produced from Zi No.5 and Xiubi No.2 was not obvious. The plantlets were developed from vigorous buds of the two seeds by elongating and induced rooting. The results indicated that the amounts of adventitious buds as well as the difference in the capacity of root differentiation of the two selected castor species was not obvious, which reveals that there exists no significant differences between the two selected castor genotypes in construction of in vitro regeneration system.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第5期484-488,F0002,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家"973"计划项目(2011CB111514)
关键词
蓖麻
胚轴
再生体系
外植体
Riciuns communis
embryo axes
plant regeneration
explants
