摘要
用甘薯品种农林17号(中紫)的叶柄分离原生质体,对其在添加各种浓度的萘乙酸和激动素的改良细胞薄层培养基中进行悬浮培养。培养1～2 d 后,原生质体再生细胞壁,培养2～3 d 后,再生细胞发生第一次分裂,持续的细胞分裂形成小愈伤组织。悬浮培养的结果表明萘乙酸和激动素的适宜浓度分别为2.0～5.0mg/L 和1.0～5.0mg/L。此后,将小愈伤组织转移到MS 固体培养基上培养,小愈伤组织迅速生长。本试验达到根的分化.
Protoplasts were isolated from young petioles of sweet potato cv.Nakamurashaki.First,protoplasts were suspension cultured in the modified cell layer medium supplemented with 1-Naphthaleneacetic acid (NAA) and kin- -etin.Cell wall reformation occurred within 1～2 days after planting,followed by first cell division at 2～3 days.Protoplast-derived colonies continued proli- feration to small calli.It was shown that the optimal concentrations of NAA and kinetin were 2.0～5.0mg/L and 1.0～5.0mg/L,respectively,during the sus- pension culture.And then the formed small calli were transferred onto solid MS (Murashige and Skoog,1962) and proliferated rapidly.Root development from protoplast-derived calli was observed.
关键词
甘薯
原生质体
分离
培养
根分化
sweet potato(Ipomoea batatas Lam.)
protoplast suspension culture
root development
