摘要
本文首次提出了偶合反应化学发光酶联免疾分析测定HRP的新方法,系统地分析和研究了偶合反应在化学发光酶联免疫分析中的应用;提出了五类偶合反应化学发光酶联免疫分析测定HRP的新体系,并对各类偶合反应化学发光体系测定的HRP灵敏度作了比较。
A method for the determination of horse radish peroxidese(HRP) and its application were investigated by coupled reaction-Chemiluminescence enzyme immunoassay(CLEIA). Five types of coupled reaction-CLEIA for determining HRP have been studied, and the sensitivity of each system to detetmine HRP is compared. The result shows that KI-H_2O_2-HRP-luminol and eosin-H_2O_2-HRP-luminol systems of CLEIA with coupled reaction have higher sensitivity.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1991年第8期895-899,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助课题
关键词
偶合反应
化学发光
酶联免疫分析
Coupled reaction
Chemiluminesence
Horse radish peroxidase
